Detailed information of TA system TA024946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 3148430..3149076
• Replicon: chromosome
• Accession: NC_014618
• Organism: Enterobacter lignolyticus SCF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E3G263
• Locus tag: ENTCL_RS14660
• Protein ID: WP_013366927.1
• Coordinates: 3148430..3148765 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ENTCL_RS14665
• Protein ID: WP_125451941.1
• Coordinates: 3148762..3149076 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTCL_RS14630	3143814..3144329	+	516	WP_013366921.1	lipoprotein	-
ENTCL_RS14635	3144530..3145258	+	729	WP_013366922.1	arginine ABC transporter ATP-binding protein ArtP	-
ENTCL_RS14640	3145276..3146007	+	732	WP_013366923.1	arginine ABC transporter substrate-binding protein	-
ENTCL_RS14645	3146014..3146730	+	717	WP_013366924.1	arginine ABC transporter permease ArtQ	-
ENTCL_RS14650	3146730..3147398	+	669	WP_013366925.1	arginine ABC transporter permease ArtM	-
ENTCL_RS14655	3147571..3148302	+	732	WP_013366926.1	ABC transporter substrate-binding protein ArtJ	-
ENTCL_RS14660	3148430..3148765	+	336	WP_013366927.1	hypothetical protein	Toxin
ENTCL_RS14665	3148762..3149076	+	315	WP_125451941.1	transcriptional regulator	Antitoxin
ENTCL_RS14670	3149084..3150556	-	1473	WP_013366929.1	two-component sensor histidine kinase	-
ENTCL_RS14675	3150553..3151275	-	723	WP_013366930.1	response regulator transcription factor	-
ENTCL_RS14680	3151417..3152016	+	600	WP_013366931.1	cytochrome b/b6 domain-containing protein	-
ENTCL_RS14685	3152027..3152797	+	771	WP_013366932.1	molybdopterin-dependent oxidoreductase	-
ENTCL_RS14690	3152827..3153081	+	255	WP_013366933.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-87)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13266.40 Da
• Isoelectric Point: 8.8722

>T24946 WP_013366927.1 NC_014618:3148430-3148765 [[Enterobacter] lignolyticus SCF1]
MDAVFVEMPPFERHRAYYLSDESFGELQQLLMINPLAGDVIKHTGGLRKLRFVDIKRGKGQRGGLRVIYYWWQSKWQFLL
FTLYNKNEMVDLTQTQCEILHQMLEQRIRGE

Nucleotide

• Length: 336 bp

>T24946 NC_014618:3148430-3148765 [[Enterobacter] lignolyticus SCF1]
ATGGACGCGGTGTTTGTCGAGATGCCCCCTTTTGAGCGGCATCGTGCTTACTATCTTAGTGATGAGTCATTTGGTGAATT
GCAGCAACTGCTGATGATCAATCCGCTTGCGGGCGATGTGATTAAGCATACGGGCGGCCTCAGAAAATTGCGCTTTGTCG
ATATCAAACGCGGCAAAGGTCAAAGAGGCGGACTTCGCGTTATCTATTACTGGTGGCAGAGCAAATGGCAGTTCCTGCTG
TTTACCCTCTATAACAAAAATGAGATGGTCGATCTGACCCAGACCCAATGTGAGATACTTCATCAAATGCTGGAACAACG
AATCCGGGGGGAATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12021.94 Da
• Isoelectric Point: 10.7083

>AT24946 WP_125451941.1 NC_014618:3148762-3149076 [[Enterobacter] lignolyticus SCF1]
IMSRDLFTELTEGMHAWQESNEGKRTLKTHRLATQDILLTAEDIRGIRRRLNLSQALFARYLHTALKTYQNWEQGVARPN
KQAMLLLRIVEKQPAALHLLATLS

Nucleotide

• Length: 315 bp

>AT24946 NC_014618:3148762-3149076 [[Enterobacter] lignolyticus SCF1]
ATAATGAGCCGGGATCTTTTTACTGAGCTTACCGAAGGAATGCACGCCTGGCAGGAGTCCAATGAAGGTAAGCGTACCCT
GAAAACACATCGTCTGGCGACGCAGGATATTCTGCTAACAGCGGAAGATATCAGAGGCATCCGCCGCCGTCTGAATCTTT
CACAGGCGTTGTTTGCGCGCTATCTGCATACCGCGCTGAAAACCTATCAGAACTGGGAACAGGGCGTCGCGAGACCGAAC
AAGCAGGCTATGCTGCTGCTGCGCATTGTGGAAAAACAACCTGCGGCGCTGCATCTGCTTGCCACTCTGAGCTAA

Structures

Toxin

• AlphaFold DB: A0A806X3J1