Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5254331..5254926 Replicon chromosome
Accession NZ_CP099798
Organism Pseudomonas aeruginosa strain PAO1-L

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag NHZ68_RS24310 Protein ID WP_003113526.1
Coordinates 5254648..5254926 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NHZ68_RS24305 Protein ID WP_003113527.1
Coordinates 5254331..5254636 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHZ68_RS24275 (NHZ68_24275) 5249444..5249734 - 291 WP_033992920.1 DUF5447 family protein -
NHZ68_RS24280 (NHZ68_24280) 5249946..5250218 - 273 WP_003115921.1 hypothetical protein -
NHZ68_RS24285 (NHZ68_24285) 5250344..5250613 + 270 WP_071536122.1 hypothetical protein -
NHZ68_RS24290 (NHZ68_24290) 5250748..5251845 + 1098 WP_169917322.1 protein phosphatase 2C domain-containing protein -
NHZ68_RS24295 (NHZ68_24295) 5251848..5252897 + 1050 WP_003113529.1 serine/threonine-protein kinase -
NHZ68_RS24300 (NHZ68_24300) 5252966..5253967 + 1002 WP_228772168.1 serine/threonine-protein kinase -
NHZ68_RS24305 (NHZ68_24305) 5254331..5254636 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
NHZ68_RS24310 (NHZ68_24310) 5254648..5254926 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NHZ68_RS24315 (NHZ68_24315) 5254979..5255107 - 129 Protein_4801 integrase -
NHZ68_RS24320 (NHZ68_24320) 5255255..5257483 + 2229 WP_003113525.1 TonB-dependent receptor -
NHZ68_RS24325 (NHZ68_24325) 5257553..5258200 - 648 WP_003095021.1 carbonate dehydratase -
NHZ68_RS24330 (NHZ68_24330) 5258262..5259500 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T249450 WP_003113526.1 NZ_CP099798:c5254926-5254648 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT249450 WP_003113527.1 NZ_CP099798:c5254636-5254331 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References