Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5195788..5196383 Replicon chromosome
Accession NZ_CP099797
Organism Pseudomonas aeruginosa strain PAO1-N

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag NHZ69_RS24090 Protein ID WP_003113526.1
Coordinates 5196105..5196383 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NHZ69_RS24085 Protein ID WP_003113527.1
Coordinates 5195788..5196093 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHZ69_RS24055 (NHZ69_24055) 5190901..5191191 - 291 WP_033992920.1 DUF5447 family protein -
NHZ69_RS24060 (NHZ69_24060) 5191403..5191675 - 273 WP_003115921.1 hypothetical protein -
NHZ69_RS24065 (NHZ69_24065) 5191801..5192070 + 270 WP_071536122.1 hypothetical protein -
NHZ69_RS24070 (NHZ69_24070) 5192205..5193302 + 1098 WP_169917322.1 protein phosphatase 2C domain-containing protein -
NHZ69_RS24075 (NHZ69_24075) 5193305..5194354 + 1050 WP_003113529.1 serine/threonine-protein kinase -
NHZ69_RS24080 (NHZ69_24080) 5194423..5195424 + 1002 WP_228772168.1 serine/threonine-protein kinase -
NHZ69_RS24085 (NHZ69_24085) 5195788..5196093 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
NHZ69_RS24090 (NHZ69_24090) 5196105..5196383 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NHZ69_RS24095 (NHZ69_24095) 5196436..5196564 - 129 Protein_4762 integrase -
NHZ69_RS24100 (NHZ69_24100) 5196712..5198940 + 2229 WP_003113525.1 TonB-dependent receptor -
NHZ69_RS24105 (NHZ69_24105) 5199010..5199657 - 648 WP_003095021.1 carbonate dehydratase -
NHZ69_RS24110 (NHZ69_24110) 5199719..5200957 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T249443 WP_003113526.1 NZ_CP099797:c5196383-5196105 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT249443 WP_003113527.1 NZ_CP099797:c5196093-5195788 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References