Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4026191..4026885 Replicon chromosome
Accession NZ_CP099754
Organism Escherichia coli strain 806883-9-2019

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag NH020_RS19410 Protein ID WP_001263491.1
Coordinates 4026191..4026589 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag NH020_RS19415 Protein ID WP_000554755.1
Coordinates 4026592..4026885 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH020_RS19380 (4021453) 4021453..4022910 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
NH020_RS19385 (4022919) 4022919..4023200 + 282 WP_022645226.1 hypothetical protein -
NH020_RS19390 (4023217) 4023217..4023726 - 510 WP_001361775.1 metal-dependent hydrolase -
NH020_RS19395 (4023788) 4023788..4024402 - 615 WP_022645225.1 peptide chain release factor H -
NH020_RS19400 (4024399) 4024399..4025538 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
NH020_RS19405 (4025729) 4025729..4026181 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
NH020_RS19410 (4026191) 4026191..4026589 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NH020_RS19415 (4026592) 4026592..4026885 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NH020_RS19420 (4026937) 4026937..4027992 - 1056 WP_022645222.1 DNA polymerase IV -
NH020_RS19425 (4028063) 4028063..4028848 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
NH020_RS19430 (4028820) 4028820..4030532 + 1713 Protein_3812 flagellar biosynthesis protein FlhA -
NH020_RS19435 (4030630) 4030630..4031403 - 774 WP_022645219.1 C40 family peptidase -
NH020_RS19440 (4031589) 4031589..4031849 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T249427 WP_001263491.1 NZ_CP099754:c4026589-4026191 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT249427 WP_000554755.1 NZ_CP099754:c4026885-4026592 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References