Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3749838..3750517 Replicon chromosome
Accession NZ_CP099754
Organism Escherichia coli strain 806883-9-2019

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag NH020_RS18095 Protein ID WP_000057541.1
Coordinates 3750215..3750517 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NH020_RS18090 Protein ID WP_000806442.1
Coordinates 3749838..3750179 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH020_RS18080 (3746081) 3746081..3747013 - 933 WP_022645289.1 glutaminase A -
NH020_RS18085 (3747276) 3747276..3749780 + 2505 WP_022645288.1 copper-exporting P-type ATPase CopA -
NH020_RS18090 (3749838) 3749838..3750179 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NH020_RS18095 (3750215) 3750215..3750517 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NH020_RS18100 (3750650) 3750650..3751444 + 795 WP_022645287.1 TraB/GumN family protein -
NH020_RS18105 (3751648) 3751648..3752127 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NH020_RS18110 (3752164) 3752164..3753816 - 1653 WP_260584470.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
NH020_RS18115 (3754034) 3754034..3755254 + 1221 WP_022645286.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T249425 WP_000057541.1 NZ_CP099754:c3750517-3750215 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT249425 WP_000806442.1 NZ_CP099754:c3750179-3749838 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References