Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4706932..4707534 Replicon chromosome
Accession NZ_CP099721
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag NH569_RS23070 Protein ID WP_000897302.1
Coordinates 4707223..4707534 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NH569_RS23065 Protein ID WP_000356397.1
Coordinates 4706932..4707222 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS23040 (4703005) 4703005..4703907 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
NH569_RS23045 (4703904) 4703904..4704539 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NH569_RS23050 (4704536) 4704536..4705465 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
NH569_RS23055 (4705681) 4705681..4705899 - 219 WP_001314326.1 CopG family transcriptional regulator -
NH569_RS23060 (4706295) 4706295..4706573 - 279 WP_001296612.1 hypothetical protein -
NH569_RS23065 (4706932) 4706932..4707222 - 291 WP_000356397.1 NadS family protein Antitoxin
NH569_RS23070 (4707223) 4707223..4707534 - 312 WP_000897302.1 hypothetical protein Toxin
NH569_RS23075 (4707763) 4707763..4708671 + 909 WP_001331553.1 alpha/beta hydrolase -
NH569_RS23080 (4708735) 4708735..4709676 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NH569_RS23085 (4709721) 4709721..4710158 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NH569_RS23090 (4710155) 4710155..4711027 - 873 WP_000920762.1 virulence factor BrkB family protein -
NH569_RS23095 (4711021) 4711021..4711620 - 600 WP_001314324.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T249337 WP_000897302.1 NZ_CP099721:c4707534-4707223 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT249337 WP_000356397.1 NZ_CP099721:c4707222-4706932 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References