Detailed information of TA system TA024933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 28584..29314
• Replicon: chromosome
• Accession: NC_014618
• Organism: Enterobacter lignolyticus SCF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E3G7Q4
• Locus tag: ENTCL_RS00130
• Protein ID: WP_013364087.1
• Coordinates: 28584..28898 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ENTCL_RS00135
• Protein ID: WP_013364088.1
• Coordinates: 28895..29314 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTCL_RS00115	24607..25944	-	1338	WP_013364084.1	D-serine transporter DsdX	-
ENTCL_RS00120	26172..27125	+	954	WP_013364085.1	DNA-binding transcriptional regulator DsdC	-
ENTCL_RS00125	27100..28284	-	1185	WP_013364086.1	multidrug efflux MFS transporter EmrD	-
ENTCL_RS00130	28584..28898	+	315	WP_013364087.1	type II toxin-antitoxin system HigB family toxin	Toxin
ENTCL_RS00135	28895..29314	+	420	WP_013364088.1	helix-turn-helix domain-containing protein	Antitoxin
ENTCL_RS23545	29462..29563	+	102	WP_125452054.1	ilvB operon leader peptide IvbL	-
ENTCL_RS00140	29668..31356	+	1689	WP_013364089.1	acetolactate synthase large subunit	-
ENTCL_RS00145	31360..31647	+	288	WP_013364090.1	acetolactate synthase small subunit	-
ENTCL_RS00150	31649..32434	-	786	WP_013364091.1	DeoR family transcriptional regulator	-
ENTCL_RS00155	32761..33681	+	921	WP_013364092.1	ribokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12267.39 Da
• Isoelectric Point: 10.1475

>T24933 WP_013364087.1 NC_014618:28584-28898 [[Enterobacter] lignolyticus SCF1]
MHLISMKAILDAVNTFPHHKEELLLLGRVIEKGNCPTPAALKKLYPTLDNFKYLDKHYIIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTDKHRAKGKK

Nucleotide

• Length: 315 bp

>T24933 NC_014618:28584-28898 [[Enterobacter] lignolyticus SCF1]
ATGCATTTAATCTCAATGAAGGCTATTTTGGATGCGGTAAATACGTTTCCGCACCACAAGGAAGAGCTACTCCTTCTGGG
GAGAGTGATCGAAAAGGGAAATTGCCCGACGCCTGCTGCACTGAAAAAGCTCTATCCAACGCTGGATAACTTCAAGTATC
TGGATAAACATTATATTATTGATATTGCAAATAATAATCTTAGGGTGGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGATAAACATCGCGCTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15499.08 Da
• Isoelectric Point: 4.9534

>AT24933 WP_013364088.1 NC_014618:28895-29314 [[Enterobacter] lignolyticus SCF1]
MMIVADAIKATQALVAAVPLLGEHPNDKDYEEALELVEYLLMNEPRSALLDIVCAKISRYEANQPEIVALRQEMESVPVG
IAVLRTLMDQHKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAIRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT24933 NC_014618:28895-29314 [[Enterobacter] lignolyticus SCF1]
ATGATGATCGTCGCTGATGCAATTAAAGCTACCCAGGCTCTTGTTGCGGCTGTTCCCTTACTGGGCGAACATCCGAACGA
CAAAGATTATGAGGAAGCCCTTGAGCTGGTTGAGTATTTGCTCATGAACGAGCCACGCAGCGCTTTGCTGGATATCGTAT
GTGCCAAAATCAGCCGCTATGAAGCGAATCAACCCGAAATAGTGGCCTTAAGGCAGGAGATGGAGTCTGTTCCTGTCGGT
ATTGCGGTTTTAAGAACCCTTATGGATCAGCACAAACTGACTATTTCTGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCCAGGGTGCTTAATGGTCAGCGTCAGCTTACCTTAAATCATATTAAAAAGCTGGCGATCCGTTTTGGCGTGTCGC
CTGCGCTTTTCATAGAATGA

Structures

Toxin

• AlphaFold DB: A0A806X9T4