Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3710852..3711546 Replicon chromosome
Accession NZ_CP099721
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag NH569_RS18160 Protein ID WP_001263500.1
Coordinates 3710852..3711250 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NH569_RS18165 Protein ID WP_000554758.1
Coordinates 3711253..3711546 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS18135 (3706217) 3706217..3706675 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
NH569_RS18140 (3706936) 3706936..3708393 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
NH569_RS18145 (3708450) 3708450..3709064 - 615 WP_000602123.1 peptide chain release factor H -
NH569_RS18150 (3709061) 3709061..3710200 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
NH569_RS18155 (3710390) 3710390..3710842 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
NH569_RS18160 (3710852) 3710852..3711250 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NH569_RS18165 (3711253) 3711253..3711546 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NH569_RS18170 (3711598) 3711598..3712653 - 1056 WP_001226168.1 DNA polymerase IV -
NH569_RS18175 (3712724) 3712724..3713509 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
NH569_RS18180 (3713481) 3713481..3715193 + 1713 Protein_3558 flagellar biosynthesis protein FlhA -
NH569_RS18185 (3715272) 3715272..3715430 + 159 WP_014639450.1 hypothetical protein -
NH569_RS18190 (3715513) 3715513..3716010 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3710852..3731205 20353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T249329 WP_001263500.1 NZ_CP099721:c3711250-3710852 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT249329 WP_000554758.1 NZ_CP099721:c3711546-3711253 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References