Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3492294..3492973 Replicon chromosome
Accession NZ_CP099721
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag NH569_RS17115 Protein ID WP_000057524.1
Coordinates 3492671..3492973 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NH569_RS17110 Protein ID WP_000806442.1
Coordinates 3492294..3492635 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS17100 (3488538) 3488538..3489470 - 933 WP_000883052.1 glutaminase A -
NH569_RS17105 (3489732) 3489732..3492236 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
NH569_RS17110 (3492294) 3492294..3492635 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NH569_RS17115 (3492671) 3492671..3492973 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NH569_RS17120 (3493106) 3493106..3493900 + 795 WP_000365177.1 TraB/GumN family protein -
NH569_RS17125 (3494104) 3494104..3494583 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NH569_RS17130 (3494607) 3494607..3495185 + 579 WP_021522671.1 hypothetical protein -
NH569_RS17135 (3495404) 3495404..3495907 + 504 WP_000667000.1 hypothetical protein -
NH569_RS17140 (3495945) 3495945..3497597 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3484983..3495907 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T249327 WP_000057524.1 NZ_CP099721:c3492973-3492671 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT249327 WP_000806442.1 NZ_CP099721:c3492635-3492294 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References