Detailed information of TA system TA249189

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27833..28254
• Replicon: plasmid unnamed
• Accession: NZ_CP099591
• Organism: Escherichia coli strain WG1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NHF37_RS22880
• Protein ID: WP_096937776.1
• Coordinates: 28129..28254 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27833..28031 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHF37_RS22850 (23763)	23763..24302	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
NHF37_RS22855 (24359)	24359..24592	+	234	WP_000005990.1	DUF905 family protein	-
NHF37_RS22860 (24658)	24658..26616	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
NHF37_RS22865 (26671)	26671..27105	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
NHF37_RS22870 (27102)	27102..27864	+	763	Protein_29	plasmid SOS inhibition protein A	-
- (27833)	27833..28031	+	199	NuclAT_0	-	Antitoxin
- (27833)	27833..28031	+	199	NuclAT_0	-	Antitoxin
- (27833)	27833..28031	+	199	NuclAT_0	-	Antitoxin
- (27833)	27833..28031	+	199	NuclAT_0	-	Antitoxin
NHF37_RS22875 (28038)	28038..28187	+	150	Protein_30	DUF5431 family protein	-
NHF37_RS22880 (28129)	28129..28254	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
NHF37_RS22885 (28555)	28555..28851	-	297	Protein_32	hypothetical protein	-
NHF37_RS22890 (28921)	28921..29127	+	207	WP_000547965.1	hypothetical protein	-
NHF37_RS22895 (29152)	29152..29439	+	288	WP_000107542.1	hypothetical protein	-
NHF37_RS22900 (29558)	29558..30379	+	822	WP_001234417.1	DUF932 domain-containing protein	-
NHF37_RS22905 (30675)	30675..31277	-	603	WP_000243701.1	transglycosylase SLT domain-containing protein	-
NHF37_RS22910 (31598)	31598..31981	+	384	WP_001151527.1	conjugal transfer relaxosome DNA-binding protein TraM	-
NHF37_RS22915 (32168)	32168..32857	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67408	67408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T249189 WP_096937776.1 NZ_CP099591:28129-28254 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 199 bp

>AT249189 NZ_CP099591:27833-28031 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin