Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3664850..3665391 Replicon chromosome
Accession NZ_CP099590
Organism Escherichia coli strain WG1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NHF37_RS17995 Protein ID WP_000615983.1
Coordinates 3665113..3665391 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NHF37_RS17990 Protein ID WP_000729703.1
Coordinates 3664850..3665110 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF37_RS17970 (3660525) 3660525..3661310 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NHF37_RS17975 (3661282) 3661282..3662994 + 1713 Protein_3538 flagellar biosynthesis protein FlhA -
NHF37_RS17980 (3663218) 3663218..3663715 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NHF37_RS17985 (3663891) 3663891..3664640 - 750 WP_000056849.1 C40 family peptidase -
NHF37_RS17990 (3664850) 3664850..3665110 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NHF37_RS17995 (3665113) 3665113..3665391 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NHF37_RS18000 (3665547) 3665547..3666287 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NHF37_RS18005 (3666258) 3666258..3667025 - 768 WP_000333380.1 class II glutamine amidotransferase -
NHF37_RS18010 (3667231) 3667231..3667809 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T249182 WP_000615983.1 NZ_CP099590:3665113-3665391 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT249182 WP_000729703.1 NZ_CP099590:3664850-3665110 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References