Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3658653..3659347 Replicon chromosome
Accession NZ_CP099590
Organism Escherichia coli strain WG1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NHF37_RS17955 Protein ID WP_001263489.1
Coordinates 3658653..3659051 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NHF37_RS17960 Protein ID WP_000554758.1
Coordinates 3659054..3659347 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3654241) 3654241..3654321 - 81 NuclAT_12 - -
- (3654241) 3654241..3654321 - 81 NuclAT_12 - -
- (3654241) 3654241..3654321 - 81 NuclAT_12 - -
- (3654241) 3654241..3654321 - 81 NuclAT_12 - -
NHF37_RS17930 (3654917) 3654917..3655375 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NHF37_RS17935 (3655636) 3655636..3657093 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NHF37_RS17940 (3657150) 3657150..3657671 - 522 Protein_3531 peptide chain release factor H -
NHF37_RS17945 (3657667) 3657667..3657873 - 207 Protein_3532 RtcB family protein -
NHF37_RS17950 (3658191) 3658191..3658643 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NHF37_RS17955 (3658653) 3658653..3659051 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NHF37_RS17960 (3659054) 3659054..3659347 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NHF37_RS17965 (3659399) 3659399..3660454 - 1056 WP_001226164.1 DNA polymerase IV -
NHF37_RS17970 (3660525) 3660525..3661310 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NHF37_RS17975 (3661282) 3661282..3662994 + 1713 Protein_3538 flagellar biosynthesis protein FlhA -
NHF37_RS17980 (3663218) 3663218..3663715 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T249181 WP_001263489.1 NZ_CP099590:c3659051-3658653 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT249181 WP_000554758.1 NZ_CP099590:c3659347-3659054 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References