Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3669974..3670515 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NHF38_RS18015 Protein ID WP_000615983.1
Coordinates 3670237..3670515 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NHF38_RS18010 Protein ID WP_000729703.1
Coordinates 3669974..3670234 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS17990 (3665649) 3665649..3666434 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NHF38_RS17995 (3666406) 3666406..3668118 + 1713 Protein_3542 flagellar biosynthesis protein FlhA -
NHF38_RS18000 (3668342) 3668342..3668839 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NHF38_RS18005 (3669015) 3669015..3669764 - 750 WP_000056849.1 C40 family peptidase -
NHF38_RS18010 (3669974) 3669974..3670234 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NHF38_RS18015 (3670237) 3670237..3670515 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NHF38_RS18020 (3670671) 3670671..3671411 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NHF38_RS18025 (3671382) 3671382..3672149 - 768 WP_000333380.1 class II glutamine amidotransferase -
NHF38_RS18030 (3672355) 3672355..3672933 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T249151 WP_000615983.1 NZ_CP099588:3670237-3670515 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT249151 WP_000729703.1 NZ_CP099588:3669974-3670234 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References