Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3663777..3664471 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NHF38_RS17975 Protein ID WP_001263489.1
Coordinates 3663777..3664175 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NHF38_RS17980 Protein ID WP_000554758.1
Coordinates 3664178..3664471 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3659365) 3659365..3659445 - 81 NuclAT_12 - -
- (3659365) 3659365..3659445 - 81 NuclAT_12 - -
- (3659365) 3659365..3659445 - 81 NuclAT_12 - -
- (3659365) 3659365..3659445 - 81 NuclAT_12 - -
NHF38_RS17950 (3660041) 3660041..3660499 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NHF38_RS17955 (3660760) 3660760..3662217 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NHF38_RS17960 (3662274) 3662274..3662795 - 522 Protein_3535 peptide chain release factor H -
NHF38_RS17965 (3662791) 3662791..3662997 - 207 Protein_3536 RtcB family protein -
NHF38_RS17970 (3663315) 3663315..3663767 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NHF38_RS17975 (3663777) 3663777..3664175 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NHF38_RS17980 (3664178) 3664178..3664471 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NHF38_RS17985 (3664523) 3664523..3665578 - 1056 WP_001226164.1 DNA polymerase IV -
NHF38_RS17990 (3665649) 3665649..3666434 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NHF38_RS17995 (3666406) 3666406..3668118 + 1713 Protein_3542 flagellar biosynthesis protein FlhA -
NHF38_RS18000 (3668342) 3668342..3668839 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T249150 WP_001263489.1 NZ_CP099588:c3664175-3663777 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT249150 WP_000554758.1 NZ_CP099588:c3664471-3664178 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References