Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 649971..650698 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag NHF38_RS03170 Protein ID WP_000550189.1
Coordinates 649971..650285 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NHF38_RS03175 Protein ID WP_000560266.1
Coordinates 650282..650698 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS03150 (646129) 646129..647115 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
NHF38_RS03155 (647194) 647194..647886 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
NHF38_RS03160 (647963) 647963..648466 - 504 WP_001333820.1 M48 family metallopeptidase -
NHF38_RS03165 (648551) 648551..649687 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NHF38_RS03170 (649971) 649971..650285 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
NHF38_RS03175 (650282) 650282..650698 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NHF38_RS03180 (650743) 650743..652761 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NHF38_RS03185 (653187) 653187..655538 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T249133 WP_000550189.1 NZ_CP099588:649971-650285 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT249133 WP_000560266.1 NZ_CP099588:650282-650698 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References