Detailed information of TA system TA024911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2609302..2609928
• Replicon: chromosome
• Accession: NC_014501
• Organism: Cyanothece sp. PCC 7822

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E0UFT5
• Locus tag: CYAN7822_RS11415
• Protein ID: WP_013322423.1
• Coordinates: 2609302..2609595 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CYAN7822_RS11420
• Protein ID: WP_013322424.1
• Coordinates: 2609620..2609928 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYAN7822_RS11385	2604482..2605801	-	1320	WP_013322417.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-
CYAN7822_RS11390	2605909..2606340	-	432	WP_083786925.1	nucleoside deaminase	-
CYAN7822_RS11395	2606411..2607415	-	1005	WP_013322419.1	hypothetical protein	-
CYAN7822_RS11400	2607580..2608149	+	570	WP_013322420.1	5-formyltetrahydrofolate cyclo-ligase	-
CYAN7822_RS11405	2608311..2608631	+	321	WP_013322421.1	hypothetical protein	-
CYAN7822_RS11410	2608831..2609292	+	462	WP_041933213.1	hypothetical protein	-
CYAN7822_RS11415	2609302..2609595	+	294	WP_013322423.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CYAN7822_RS11420	2609620..2609928	+	309	WP_013322424.1	HigA family addiction module antidote protein	Antitoxin
CYAN7822_RS11425	2610018..2610740	-	723	WP_013322425.1	UMP kinase	-
CYAN7822_RS11430	2610827..2611735	-	909	WP_013322426.1	agmatinase	-
CYAN7822_RS11435	2611829..2612782	-	954	WP_013322427.1	DNA (cytosine-5-)-methyltransferase	-
CYAN7822_RS11440	2612859..2613887	-	1029	WP_013322428.1	isochorismatase	-
CYAN7822_RS11445	2614043..2614363	-	321	WP_013322429.1	DUF3155 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11370.89 Da
• Isoelectric Point: 5.8561

>T24911 WP_013322423.1 NC_014501:2609302-2609595 [Gloeothece verrucosa PCC 7822]
MIRSFKNQGTEDIFNGRDTKLARKICPQNLWDVVARKLDQIDSVKFLDELRVPPGNKLEPLKGERLGQYSIRINDQYRVC
FTWTDDSPTDVEIVDYH

Nucleotide

• Length: 294 bp

>T24911 NC_014501:2609302-2609595 [Gloeothece verrucosa PCC 7822]
ATGATTCGCTCTTTCAAAAATCAAGGAACTGAAGATATTTTTAATGGGAGAGATACTAAACTAGCCAGAAAAATCTGCCC
TCAAAATTTGTGGGATGTAGTAGCTAGAAAACTCGATCAAATTGACTCTGTAAAATTTTTAGATGAACTCCGGGTTCCTC
CTGGAAACAAACTAGAACCTTTAAAAGGAGAGCGCTTAGGACAATATAGTATTAGAATCAATGATCAATATCGAGTTTGT
TTTACTTGGACTGATGATAGTCCTACCGATGTAGAAATCGTTGATTATCATTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11997.01 Da
• Isoelectric Point: 6.0854

>AT24911 WP_013322424.1 NC_014501:2609620-2609928 [Gloeothece verrucosa PCC 7822]
MINIPTHRIPTHPGEMLLKEFLEPLAITQEELAEKLYISSEEIKELVKGTQGMKPRLALRLAKFWGMSPEFWLNLQLRWD
IYHIEQLEASDLAKIHPYSERV

Nucleotide

• Length: 309 bp

>AT24911 NC_014501:2609620-2609928 [Gloeothece verrucosa PCC 7822]
ATGATTAATATTCCTACTCATAGAATTCCTACTCATCCAGGTGAAATGCTTTTAAAAGAATTTTTAGAACCTTTGGCAAT
CACTCAGGAAGAATTAGCCGAAAAACTCTATATTTCCTCTGAAGAAATCAAGGAATTGGTCAAAGGTACTCAGGGAATGA
AGCCGCGTTTAGCTTTGCGTTTGGCTAAATTTTGGGGGATGTCTCCTGAGTTTTGGCTTAATCTCCAGTTGCGCTGGGAT
ATCTATCACATAGAGCAACTAGAAGCTTCTGATCTAGCAAAAATTCATCCCTATTCAGAACGGGTTTAA

Structures

Toxin

• AlphaFold DB: E0UFT5