Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 4772811..4773571 | Replicon | chromosome |
| Accession | NZ_CP099388 | ||
| Organism | Citrobacter braakii strain RHB03-E1-C02 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7L6U900 |
| Locus tag | NFJ30_RS22590 | Protein ID | WP_019077936.1 |
| Coordinates | 4772811..4773122 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | NFJ30_RS22595 | Protein ID | WP_141876133.1 |
| Coordinates | 4773119..4773571 (+) | Length | 151 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NFJ30_RS22560 (NFJ30_22550) | 4768478..4769380 | + | 903 | WP_003028691.1 | formate dehydrogenase O subunit beta | - |
| NFJ30_RS22565 (NFJ30_22555) | 4769377..4770012 | + | 636 | WP_003028686.1 | formate dehydrogenase cytochrome b556 subunit | - |
| NFJ30_RS22570 (NFJ30_22560) | 4770009..4770938 | + | 930 | WP_003028685.1 | formate dehydrogenase accessory protein FdhE | - |
| NFJ30_RS22575 (NFJ30_22565) | 4770975..4771349 | - | 375 | WP_016155182.1 | type II toxin-antitoxin system VapC family toxin | - |
| NFJ30_RS22580 (NFJ30_22570) | 4771349..4771591 | - | 243 | WP_016155181.1 | CopG family transcriptional regulator | - |
| NFJ30_RS22585 (NFJ30_22575) | 4771797..4772726 | + | 930 | WP_169330465.1 | alpha/beta hydrolase | - |
| NFJ30_RS22590 (NFJ30_22580) | 4772811..4773122 | + | 312 | WP_019077936.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| NFJ30_RS22595 (NFJ30_22585) | 4773119..4773571 | + | 453 | WP_141876133.1 | helix-turn-helix domain-containing protein | Antitoxin |
| NFJ30_RS22600 (NFJ30_22590) | 4773589..4774530 | - | 942 | WP_003825286.1 | fatty acid biosynthesis protein FabY | - |
| NFJ30_RS22605 (NFJ30_22595) | 4774575..4775012 | - | 438 | WP_016157874.1 | D-aminoacyl-tRNA deacylase | - |
| NFJ30_RS22610 (NFJ30_22600) | 4775009..4775881 | - | 873 | WP_019077938.1 | virulence factor BrkB family protein | - |
| NFJ30_RS22615 (NFJ30_22605) | 4775875..4776474 | - | 600 | WP_016155174.1 | glucose-1-phosphatase | - |
| NFJ30_RS22620 (NFJ30_22610) | 4776579..4777382 | - | 804 | WP_016155173.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
| NFJ30_RS22625 (NFJ30_22615) | 4777416..4778312 | - | 897 | WP_103928802.1 | sugar kinase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12502.67 Da Isoelectric Point: 9.9988
>T248842 WP_019077936.1 NZ_CP099388:4772811-4773122 [Citrobacter braakii]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE
Download Length: 312 bp
Antitoxin
Download Length: 151 a.a. Molecular weight: 16998.39 Da Isoelectric Point: 5.9259
>AT248842 WP_141876133.1 NZ_CP099388:4773119-4773571 [Citrobacter braakii]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE
Download Length: 453 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|