Detailed information of TA system TA248780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3029925..3030585
• Replicon: chromosome
• Accession: NZ_CP099382
• Organism: Citrobacter braakii strain RHB14-SO-C08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: NFJ83_RS14785
• Protein ID: WP_053390179.1
• Coordinates: 3030232..3030585 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NFJ83_RS14780
• Protein ID: WP_103283718.1
• Coordinates: 3029925..3030227 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NFJ83_RS14765 (3026593)	3026593..3027855	+	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-
NFJ83_RS14770 (3028537)	3028537..3029424	-	888	WP_103283719.1	integrase domain-containing protein	-
NFJ83_RS14775 (3029688)	3029688..3029858	+	171	WP_233896717.1	hypothetical protein	-
NFJ83_RS14780 (3029925)	3029925..3030227	-	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
NFJ83_RS14785 (3030232)	3030232..3030585	-	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NFJ83_RS14790 (3031084)	3031084..3031305	-	222	WP_103283717.1	helix-turn-helix transcriptional regulator	-
NFJ83_RS14795 (3031302)	3031302..3031769	-	468	WP_103283716.1	hypothetical protein	-
NFJ83_RS14800 (3032150)	3032150..3033754	-	1605	WP_103283715.1	LA2681 family HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T248780 WP_053390179.1 NZ_CP099382:c3030585-3030232 [Citrobacter braakii]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T248780 NZ_CP099382:c3030585-3030232 [Citrobacter braakii]
GTGTGGACGATTAAAACAACGGATATGTTTGATCACTGGTTTTCCTCGCTCAATGATATCGATCGGGCCAGCGTGCTTGC
AGCGCTGTTGGTCTTGCGGGAAAAGGGGCCAGGTTTGTCCAGACCTTATGCCGATACGCTTAGAGGCTCCCGCTTTAGCA
ACATGAAAGAGTTGCGCATTCAGAGCCGGGGCGATCCGATACGGGCATTTTTCGCTTTTGATCCAGCCCGTACCGGCATT
GTACTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGATCCCGGTTGCAGATCGGGAATTCAC
GAACTGGCTGAACACAATAAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT248780 WP_103283718.1 NZ_CP099382:c3030227-3029925 [Citrobacter braakii]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT248780 NZ_CP099382:c3030227-3029925 [Citrobacter braakii]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAACATTCACCTTGCCGAACTGCGCGAGAAAGTGCAGAAAACGCAGGTCGAAATGGCGAAGGCTCTGGGGATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACCGGGGCGCGACCTGAAGCTTTCCACGCTGAAACGCTACGTTGAAGCGACC
GGCGGCAAGCTACGCCTTGATATTGAACTGCCGGATGGTTCTCACTATGGATTTGTACTATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S535