Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 35702..36351 Replicon chromosome
Accession NZ_CP099378
Organism Citrobacter braakii strain RHB21-E1-C04

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag NFK10_RS00180 Protein ID WP_016157800.1
Coordinates 35702..36043 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag NFK10_RS00185 Protein ID WP_016157799.1
Coordinates 36052..36351 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK10_RS00165 (32138) 32138..33466 + 1329 WP_016155042.1 MFS transporter -
NFK10_RS00170 (33609) 33609..35000 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
NFK10_RS00175 (35149) 35149..35601 + 453 WP_016157801.1 DUF1198 family protein -
NFK10_RS00180 (35702) 35702..36043 + 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFK10_RS00185 (36052) 36052..36351 + 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
NFK10_RS00190 (36470) 36470..37654 + 1185 WP_016157798.1 purine ribonucleoside efflux pump NepI -
NFK10_RS00195 (37710) 37710..39092 - 1383 WP_016157797.1 glycoside hydrolase family 1 protein -
NFK10_RS00200 (39185) 39185..39478 - 294 WP_016155038.1 YicS family protein -
NFK10_RS00205 (39609) 39609..40025 + 417 WP_016157796.1 GNAT family N-acetyltransferase -
NFK10_RS00210 (40197) 40197..41015 + 819 WP_016157795.1 lipoprotein NlpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T248740 WP_016157800.1 NZ_CP099378:35702-36043 [Citrobacter braakii]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT248740 WP_016157799.1 NZ_CP099378:36052-36351 [Citrobacter braakii]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References