Detailed information of TA system TA024873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 784281..784908
• Replicon: chromosome
• Accession: NC_014394
• Organism: Gallionella capsiferriformans ES-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D9SDM2
• Locus tag: GALF_RS03725
• Protein ID: WP_013292720.1
• Coordinates: 784281..784610 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GALF_RS03730
• Protein ID: WP_013292721.1
• Coordinates: 784600..784908 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GALF_RS14790	779526..780866	-	1341	WP_013292712.1	integrase family protein	-
GALF_RS03695	781246..781506	+	261	WP_013292713.1	ribbon-helix-helix domain-containing protein	-
GALF_RS03700	781506..781781	+	276	WP_013292714.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GALF_RS03705	781844..782065	+	222	WP_013292715.1	RNA-binding S4 domain-containing protein	-
GALF_RS03710	782215..782886	-	672	WP_013292716.1	Crp/Fnr family transcriptional regulator	-
GALF_RS03715	783008..783475	+	468	WP_013292717.1	cytochrome c	-
GALF_RS03720	783753..783980	-	228	WP_013292718.1	hypothetical protein	-
GALF_RS03725	784281..784610	+	330	WP_013292720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GALF_RS03730	784600..784908	+	309	WP_013292721.1	helix-turn-helix domain-containing protein	Antitoxin
GALF_RS03735	785300..786301	+	1002	WP_013292722.1	NERD domain-containing protein	-
GALF_RS03740	786345..786644	+	300	WP_013292723.1	GIY-YIG nuclease family protein	-
GALF_RS03750	786817..787098	+	282	Protein_728	integration host factor subunit beta	-
GALF_RS03755	787119..787559	+	441	WP_013292724.1	hypothetical protein	-
GALF_RS03760	787619..788026	+	408	WP_013292725.1	hypothetical protein	-
GALF_RS03765	788402..788665	+	264	WP_013292726.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	714838..785098	70260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12495.60 Da
• Isoelectric Point: 10.3872

>T24873 WP_013292720.1 NC_014394:784281-784610 [Gallionella capsiferriformans ES-2]
MNWRIIYFSERVRSDVLALPPGIRADYLRLAELMEEHGADLRMPHSKAMGSGLFELRPKGPEGIGRVFYCTQVGQMIVVL
HSFVKKTQRTPNNELDIATRRLKEVKNAK

Nucleotide

• Length: 330 bp

>T24873 NC_014394:784281-784610 [Gallionella capsiferriformans ES-2]
ATGAACTGGCGGATAATTTACTTTTCTGAACGTGTGCGATCTGATGTGTTGGCCTTGCCTCCCGGCATCCGAGCCGACTA
TCTGCGTCTGGCTGAACTGATGGAAGAACATGGTGCCGACTTGAGGATGCCTCACTCCAAGGCAATGGGAAGTGGTCTGT
TTGAATTACGTCCCAAAGGCCCGGAGGGAATCGGTCGTGTGTTCTACTGTACTCAGGTAGGGCAGATGATTGTTGTGCTT
CATTCATTCGTCAAGAAGACACAAAGAACACCGAATAATGAGTTGGATATTGCGACCCGTCGCTTGAAGGAGGTTAAAAA
TGCAAAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11347.82 Da
• Isoelectric Point: 6.9780

>AT24873 WP_013292721.1 NC_014394:784600-784908 [Gallionella capsiferriformans ES-2]
MQNERFNPVAHDDAFVAQMLSDVETKHAYDELEEEYVALHAVLRARREAGLTQSEIAQRMGTTTSAVSRLESSLISQKHS
PSFSTLKKYAHACGKKLVISLA

Nucleotide

• Length: 309 bp

>AT24873 NC_014394:784600-784908 [Gallionella capsiferriformans ES-2]
ATGCAAAATGAACGATTCAATCCTGTTGCTCACGATGATGCGTTTGTTGCTCAGATGTTGAGTGATGTTGAAACAAAACA
TGCCTATGATGAGCTTGAGGAAGAGTATGTGGCTCTGCATGCTGTCTTGCGTGCTCGTCGTGAGGCTGGCTTGACACAGT
CCGAGATTGCTCAACGCATGGGTACAACAACTAGTGCAGTGTCTCGTCTGGAGTCATCTCTGATCAGTCAGAAGCATTCG
CCTAGTTTTTCGACATTGAAAAAATATGCTCATGCTTGCGGGAAAAAATTGGTGATCAGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: D9SDM2