Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4790072..4790832 Replicon chromosome
Accession NZ_CP099376
Organism Citrobacter braakii strain RHB23-SO-C04

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6U900
Locus tag NFK21_RS22755 Protein ID WP_019077936.1
Coordinates 4790072..4790383 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NFK21_RS22760 Protein ID WP_141876133.1
Coordinates 4790380..4790832 (+) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK21_RS22725 (4785739) 4785739..4786641 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
NFK21_RS22730 (4786638) 4786638..4787273 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFK21_RS22735 (4787270) 4787270..4788199 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFK21_RS22740 (4788236) 4788236..4788610 - 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin -
NFK21_RS22745 (4788610) 4788610..4788852 - 243 WP_016155181.1 CopG family transcriptional regulator -
NFK21_RS22750 (4789058) 4789058..4789987 + 930 WP_137351792.1 alpha/beta hydrolase -
NFK21_RS22755 (4790072) 4790072..4790383 + 312 WP_019077936.1 type II toxin-antitoxin system HigB family toxin Toxin
NFK21_RS22760 (4790380) 4790380..4790832 + 453 WP_141876133.1 helix-turn-helix domain-containing protein Antitoxin
NFK21_RS22765 (4790850) 4790850..4791791 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NFK21_RS22770 (4791836) 4791836..4792273 - 438 WP_149382140.1 D-aminoacyl-tRNA deacylase -
NFK21_RS22775 (4792270) 4792270..4793142 - 873 WP_279266673.1 virulence factor BrkB family protein -
NFK21_RS22780 (4793136) 4793136..4793735 - 600 WP_279266674.1 glucose-1-phosphatase -
NFK21_RS22785 (4793840) 4793840..4794643 - 804 WP_016155173.1 DeoR/GlpR family DNA-binding transcription regulator -
NFK21_RS22790 (4794677) 4794677..4795573 - 897 WP_104651901.1 sugar kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(95-148)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12502.67 Da        Isoelectric Point: 9.9988

>T248727 WP_019077936.1 NZ_CP099376:4790072-4790383 [Citrobacter braakii]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Download         Length: 312 bp

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16998.39 Da        Isoelectric Point: 5.9259

>AT248727 WP_141876133.1 NZ_CP099376:4790380-4790832 [Citrobacter braakii]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Download         Length: 453 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6U900

Antitoxin

Source ID Structure

References