Detailed information of TA system TA024870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-couple_hipB
• Location: 897980..898656
• Replicon: chromosome
• Accession: NC_014391
• Organism: Micromonospora aurantiaca ATCC 27029

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D9TB09
• Locus tag: MICAU_RS04490
• Protein ID: WP_013284103.1
• Coordinates: 898294..898656 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MICAU_RS04485
• Protein ID: WP_013284102.1
• Coordinates: 897980..898297 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MICAU_RS04445	893170..893805	+	636	WP_013284094.1	septum formation initiator family protein	-
MICAU_RS04450	893829..894386	+	558	WP_013284095.1	DUF501 domain-containing protein	-
MICAU_RS04455	894388..894897	+	510	WP_013284096.1	amino-acid N-acetyltransferase	-
MICAU_RS04460	894940..895869	+	930	WP_013284097.1	Ppx/GppA family phosphatase	-
MICAU_RS04465	895972..896151	-	180	WP_013284098.1	hypothetical protein	-
MICAU_RS04470	896252..896539	+	288	WP_013284099.1	hypothetical protein	-
MICAU_RS04475	896603..896935	+	333	WP_041798804.1	PadR family transcriptional regulator	-
MICAU_RS04480	896932..897897	+	966	WP_013284101.1	hypothetical protein	-
MICAU_RS04485	897980..898297	-	318	WP_013284102.1	helix-turn-helix domain-containing protein	Antitoxin
MICAU_RS04490	898294..898656	-	363	WP_013284103.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MICAU_RS04495	898834..899601	+	768	WP_013284104.1	dienelactone hydrolase family protein	-
MICAU_RS04500	899621..900334	+	714	WP_013284105.1	uracil-DNA glycosylase	-
MICAU_RS04505	900355..901086	+	732	WP_013284106.1	DUF4129 domain-containing protein	-
MICAU_RS04510	901083..901667	+	585	WP_013284107.1	hypothetical protein	-
MICAU_RS04515	901667..902665	+	999	WP_013284108.1	MoxR family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13551.40 Da
• Isoelectric Point: 6.0857

>T24870 WP_013284103.1 NC_014391:c898656-898294 [Micromonospora aurantiaca ATCC 27029]
VVAGDWDVYLVDEVRQWLDTLDASAHARVVQAIDLLAEHGPGLGRPLVDTIHGSSMANLKELRPGTVRILFAFDPWRSSI
LLVAGDKSGRWKTWYQEAIPVAEHRYELYLKDRAREEGKA

Nucleotide

• Length: 363 bp

>T24870 NC_014391:c898656-898294 [Micromonospora aurantiaca ATCC 27029]
GTGGTGGCTGGAGACTGGGACGTCTACCTGGTCGACGAGGTGCGGCAGTGGCTCGACACTCTCGACGCGTCCGCCCATGC
GCGAGTGGTGCAGGCGATCGACCTCCTGGCCGAGCACGGACCAGGACTCGGACGACCCCTTGTCGACACCATCCACGGTT
CGTCGATGGCGAACTTGAAGGAGCTCCGGCCGGGCACCGTGCGTATCCTTTTCGCGTTCGACCCGTGGCGCTCGAGCATC
CTCCTGGTCGCCGGCGACAAGTCCGGCCGGTGGAAGACGTGGTATCAGGAGGCCATTCCCGTCGCCGAGCACCGCTACGA
GCTCTACCTCAAGGACCGGGCACGAGAGGAGGGGAAGGCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11399.80 Da
• Isoelectric Point: 6.7267

>AT24870 WP_013284102.1 NC_014391:c898297-897980 [Micromonospora aurantiaca ATCC 27029]
MSDYSRWQDIRADHVARVGGEDAVDAGKQELLAEVIGHRLAEVRRARGLTQQQVADRMGVTKGRVSQIEQGKISGQEVVA
RFAAALGGRLHQAIYFDDGDIAAIA

Nucleotide

• Length: 318 bp

>AT24870 NC_014391:c898297-897980 [Micromonospora aurantiaca ATCC 27029]
ATGAGCGACTACTCCCGGTGGCAGGACATCCGCGCCGACCACGTCGCCCGGGTCGGCGGGGAAGATGCGGTCGACGCGGG
TAAGCAGGAACTCCTCGCCGAGGTCATCGGTCACCGCCTCGCGGAGGTACGGCGAGCGCGCGGCCTGACCCAGCAGCAGG
TCGCCGACCGGATGGGCGTCACCAAGGGACGGGTCTCCCAGATCGAGCAGGGAAAGATCTCCGGACAGGAAGTGGTGGCC
CGGTTCGCCGCGGCGCTGGGTGGCCGGCTCCACCAGGCGATCTACTTCGACGACGGTGACATCGCCGCCATCGCCTGA

Structures

Toxin

• AlphaFold DB: D9TB09