Detailed information of TA system TA024857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-Phd
• Location: 2353705..2354207
• Replicon: chromosome
• Accession: NC_014364
• Organism: Spirochaeta smaragdinae DSM 11293

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E1R6Y9
• Locus tag: SPIRS_RS11105
• Protein ID: WP_013254779.1
• Coordinates: 2353947..2354207 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E1R6Y8
• Locus tag: SPIRS_RS11100
• Protein ID: WP_013254778.1
• Coordinates: 2353705..2353947 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPIRS_RS11060	2348796..2349302	+	507	WP_013254769.1	phage terminase small subunit P27 family	-
SPIRS_RS11065	2349299..2350480	+	1182	Protein_2177	terminase large subunit	-
SPIRS_RS11070	2350705..2351235	+	531	WP_013254771.1	DinB family protein	-
SPIRS_RS11075	2351631..2351951	+	321	WP_013254772.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SPIRS_RS11080	2351948..2352244	+	297	WP_013254773.1	helix-turn-helix domain-containing protein	-
SPIRS_RS11085	2352258..2352521	+	264	WP_013254774.1	DUF2442 domain-containing protein	-
SPIRS_RS22135	2352585..2352845	+	261	WP_095522521.1	BrnT family toxin	-
SPIRS_RS11090	2352850..2353065	+	216	WP_013254776.1	BrnA antitoxin family protein	-
SPIRS_RS11095	2353106..2353357	+	252	WP_013254777.1	CopG family transcriptional regulator	-
SPIRS_RS11100	2353705..2353947	+	243	WP_013254778.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPIRS_RS11105	2353947..2354207	+	261	WP_013254779.1	Txe/YoeB family addiction module toxin	Toxin
SPIRS_RS11110	2354626..2355288	-	663	WP_083771478.1	IS3 family transposase	-
SPIRS_RS11115	2355458..2355760	-	303	WP_171814766.1	transposase	-
SPIRS_RS11120	2356339..2357256	+	918	WP_013254781.1	class A beta-lactamase, subclass A2	-
SPIRS_RS11125	2357319..2358515	+	1197	WP_013254782.1	IS256 family transposase	-
SPIRS_RS11130	2358608..2358955	-	348	WP_013254783.1	TIGR04076 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2329685..2359336	29651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10200.94 Da
• Isoelectric Point: 9.5958

>T24857 WP_013254779.1 NC_014364:2353947-2354207 [Sediminispirochaeta smaragdinae DSM 11293]
MYTLLFTKQAQKDAKKLKSSNLKAKAEQVLEILKADPFCDYPPYEKLLGDLTGSFSRRINIQYRIVYQVLEDEKIVKVLR
MWTHYE

Nucleotide

• Length: 261 bp

>T24857 NC_014364:2353947-2354207 [Sediminispirochaeta smaragdinae DSM 11293]
ATGTATACATTACTATTTACAAAACAGGCTCAAAAAGATGCAAAGAAATTAAAATCATCAAATTTAAAGGCGAAAGCAGA
ACAAGTATTAGAGATCCTTAAAGCTGACCCATTTTGTGATTACCCACCTTATGAGAAATTGCTTGGTGATTTAACAGGTT
CATTTTCGCGTAGAATCAATATCCAATATCGAATAGTTTATCAGGTCTTAGAAGATGAAAAAATTGTGAAAGTGTTAAGA
ATGTGGACTCATTATGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9040.03 Da
• Isoelectric Point: 3.9947

>AT24857 WP_013254778.1 NC_014364:2353705-2353947 [Sediminispirochaeta smaragdinae DSM 11293]
MSSINVTNARKDLYNLVQRVNSTHEPVEITGKESNAILIGEEDWRSIQETLFLSSIPGMRESIIEGMNTSVDDLSDELDW

Nucleotide

• Length: 243 bp

>AT24857 NC_014364:2353705-2353947 [Sediminispirochaeta smaragdinae DSM 11293]
ATGAGTAGTATTAATGTTACAAATGCAAGAAAAGACCTGTATAATTTAGTACAAAGAGTTAATTCGACTCATGAACCTGT
AGAAATAACTGGAAAAGAATCAAATGCTATTTTGATAGGAGAAGAAGATTGGCGGAGTATTCAAGAAACACTTTTTCTAT
CATCAATTCCTGGTATGCGAGAATCCATAATTGAAGGTATGAATACGTCTGTCGATGATTTATCAGATGAATTGGATTGG
TAA

Structures

Toxin

• AlphaFold DB: E1R6Y9

Antitoxin

• AlphaFold DB: E1R6Y8