Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2209873..2210435 Replicon chromosome
Accession NZ_CP099331
Organism Escherichia fergusonii strain RHB44-C03

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag NFK96_RS10635 Protein ID WP_000605673.1
Coordinates 2210157..2210435 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag NFK96_RS10630 Protein ID WP_000781370.1
Coordinates 2209873..2210157 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK96_RS10615 (2205232) 2205232..2208279 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
NFK96_RS10620 (2208292) 2208292..2209176 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
NFK96_RS10625 (2209169) 2209169..2209822 + 654 WP_279284723.1 formate dehydrogenase-N subunit gamma -
NFK96_RS10630 (2209873) 2209873..2210157 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
NFK96_RS10635 (2210157) 2210157..2210435 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFK96_RS10640 (2210621) 2210621..2211631 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
NFK96_RS10645 (2211766) 2211766..2213463 - 1698 WP_046082348.1 malate dehydrogenase -
NFK96_RS10650 (2213620) 2213620..2213757 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
NFK96_RS10655 (2213859) 2213859..2214074 - 216 WP_000495766.1 biofilm-dependent modulation protein -
NFK96_RS10660 (2214418) 2214418..2214849 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T248496 WP_000605673.1 NZ_CP099331:c2210435-2210157 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT248496 WP_000781370.1 NZ_CP099331:c2210157-2209873 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References