Detailed information of TA system TA024840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2859031..2859618
• Replicon: chromosome
• Accession: NC_014315
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D8KAN3
• Locus tag: NWAT_RS13095
• Protein ID: WP_013221528.1
• Coordinates: 2859340..2859618 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NWAT_RS13090
• Protein ID: WP_013221527.1
• Coordinates: 2859031..2859327 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS13060	2854424..2856168	-	1745	Protein_2598	amidohydrolase	-
NWAT_RS17125	2856223..2856390	-	168	WP_013221521.1	hypothetical protein	-
NWAT_RS13065	2856518..2857234	+	717	WP_013221522.1	site-specific DNA-methyltransferase	-
NWAT_RS13070	2857421..2857738	-	318	WP_013221523.1	hypothetical protein	-
NWAT_RS13075	2857735..2858013	-	279	WP_013221524.1	BrnT family toxin	-
NWAT_RS13080	2858194..2858508	-	315	WP_013221525.1	HigA family addiction module antidote protein	-
NWAT_RS16345	2858519..2858800	-	282	WP_013221526.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NWAT_RS13090	2859031..2859327	-	297	WP_013221527.1	HigA family addiction module antidote protein	Antitoxin
NWAT_RS13095	2859340..2859618	-	279	WP_013221528.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NWAT_RS13100	2860047..2860241	+	195	WP_013221529.1	type II toxin-antitoxin system VapB family antitoxin	-
NWAT_RS13105	2860238..2860639	+	402	WP_013221530.1	PIN domain nuclease	-
NWAT_RS13110	2860693..2861058	-	366	WP_095522190.1	integrase core domain-containing protein	-
NWAT_RS13115	2861113..2861646	-	534	WP_013219491.1	transposase	-
NWAT_RS17130	2861660..2861971	-	312	WP_013220071.1	IS630 transposase-related protein	-
NWAT_RS13130	2862488..2862703	-	216	WP_013221532.1	hypothetical protein	-
NWAT_RS13140	2863260..2863916	-	657	WP_013221533.1	hypothetical protein	-
NWAT_RS13145	2864192..2864485	-	294	WP_013221534.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2773950..2997798	223848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10452.75 Da
• Isoelectric Point: 8.5629

>T24840 WP_013221528.1 NC_014315:c2859618-2859340 [Nitrosococcus watsonii C-113]
MIRSFAHKGMERFYQTGSKSGIQAKHATQLRLILSNLDQAEGSEDMNLPGLGLHELKGNREGVWAVKVSGNWRVTFRFVG
RDAETVNYEDYH

Nucleotide

• Length: 279 bp

>T24840 NC_014315:c2859618-2859340 [Nitrosococcus watsonii C-113]
ATGATTAGGAGTTTTGCTCACAAAGGCATGGAACGGTTTTACCAAACTGGTAGCAAGTCGGGCATTCAAGCGAAACATGC
TACGCAACTGCGGCTAATCCTCTCTAACCTGGATCAAGCGGAAGGTTCGGAGGACATGAATTTGCCCGGTCTTGGATTAC
ACGAACTTAAGGGTAATCGTGAGGGTGTTTGGGCGGTTAAGGTCAGTGGCAACTGGCGCGTGACGTTTCGCTTTGTGGGC
CGTGATGCAGAAACTGTGAATTACGAAGATTATCACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10991.78 Da
• Isoelectric Point: 10.0133

>AT24840 WP_013221527.1 NC_014315:c2859327-2859031 [Nitrosococcus watsonii C-113]
MNMHNPAHPGEILRELVIEPLGLSVTEAARHLSISRKTLSKVLNGRGVVTPEMALRLEMAFGKPNAAHWLRLQNAYDLWQ
TRQHCADIHVTPVKTHVA

Nucleotide

• Length: 297 bp

>AT24840 NC_014315:c2859327-2859031 [Nitrosococcus watsonii C-113]
ATGAATATGCATAATCCCGCTCATCCAGGCGAGATTCTAAGAGAACTGGTAATTGAGCCTTTGGGGCTATCGGTCACTGA
GGCTGCCCGTCATTTGAGTATAAGTCGAAAGACTCTTTCCAAGGTACTCAACGGTCGTGGTGTAGTTACGCCGGAGATGG
CGTTGCGGCTTGAAATGGCATTCGGCAAGCCGAATGCCGCTCACTGGCTCAGGCTGCAGAACGCCTATGATCTCTGGCAA
ACTCGTCAGCATTGCGCCGATATTCACGTTACGCCTGTGAAAACCCATGTTGCTTAA

Structures

Toxin

• AlphaFold DB: D8KAN3