Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 71150..71796 Replicon chromosome
Accession NZ_CP099308
Organism Enterobacter roggenkampii strain RHB21-E1-C01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag NFK08_RS00315 Protein ID WP_045907956.1
Coordinates 71446..71796 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B2S8A5
Locus tag NFK08_RS00310 Protein ID WP_045337168.1
Coordinates 71150..71449 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK08_RS00280 (NFK08_00280) 66552..67511 - 960 WP_023345179.1 DUF523 and DUF1722 domain-containing protein -
NFK08_RS00285 (NFK08_00285) 67614..68024 - 411 WP_008500117.1 hydroxyisourate hydrolase -
NFK08_RS00290 (NFK08_00290) 68124..68849 - 726 WP_008500116.1 MerR family transcriptional regulator -
NFK08_RS00295 (NFK08_00295) 68967..69491 + 525 WP_008500115.1 lipocalin family protein -
NFK08_RS00300 (NFK08_00300) 69570..70616 + 1047 WP_219284395.1 class I SAM-dependent methyltransferase -
NFK08_RS00305 (NFK08_00305) 70683..71120 + 438 WP_045337169.1 acetyltransferase -
NFK08_RS00310 (NFK08_00310) 71150..71449 - 300 WP_045337168.1 XRE family transcriptional regulator Antitoxin
NFK08_RS00315 (NFK08_00315) 71446..71796 - 351 WP_045907956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFK08_RS00320 (NFK08_00320) 72225..72566 - 342 WP_219284394.1 SymE family type I addiction module toxin -
NFK08_RS00325 (NFK08_00325) 72695..72856 + 162 WP_219284393.1 YlcI/YnfO family protein -
NFK08_RS00330 (NFK08_00330) 72928..75015 + 2088 WP_248199279.1 ATP-binding domain-containing protein -
NFK08_RS00335 (NFK08_00335) 75012..75710 + 699 WP_219284392.1 DUF2290 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13568.61 Da        Isoelectric Point: 9.3787

>T248370 WP_045907956.1 NZ_CP099308:c71796-71446 [Enterobacter roggenkampii]
MWTVRFGKVFEQWLFEQEEGLQDKVLADLLNLQHYGPRLPRPYADTVKGSRYKHMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKSNDKTFYEKMIRIADAEFSLHLTSQEAAK

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11121.06 Da        Isoelectric Point: 6.9836

>AT248370 WP_045337168.1 NZ_CP099308:c71449-71150 [Enterobacter roggenkampii]
VKTLNEVIASRSPESQQRIKEMAEEMILETGLQLLREELMLSQKKVADLMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S8A5

References