Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2359492..2360054 Replicon chromosome
Accession NZ_CP099303
Organism Citrobacter freundii strain RHB02-E1-C01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag NFJ16_RS11220 Protein ID WP_032936429.1
Coordinates 2359776..2360054 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QC60
Locus tag NFJ16_RS11215 Protein ID WP_003836231.1
Coordinates 2359492..2359776 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ16_RS11200 (2357183) 2357183..2358067 + 885 WP_003833024.1 formate dehydrogenase N subunit beta -
NFJ16_RS11205 (2358060) 2358060..2358716 + 657 WP_003020054.1 formate dehydrogenase-N subunit gamma -
NFJ16_RS11210 (2358846) 2358846..2359448 + 603 WP_032935729.1 inorganic diphosphatase -
NFJ16_RS11215 (2359492) 2359492..2359776 - 285 WP_003836231.1 HigA family addiction module antitoxin Antitoxin
NFJ16_RS11220 (2359776) 2359776..2360054 - 279 WP_032936429.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFJ16_RS11225 (2360275) 2360275..2361990 - 1716 WP_032935731.1 ABC-F family ATP-binding cassette domain-containing protein -
NFJ16_RS11230 (2362300) 2362300..2363997 - 1698 WP_003020042.1 malate dehydrogenase -
NFJ16_RS11235 (2364177) 2364177..2364317 - 141 WP_003020039.1 stationary-phase-induced ribosome-associated protein -
NFJ16_RS11240 (2364545) 2364545..2364976 + 432 WP_003020036.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10667.30 Da        Isoelectric Point: 9.8965

>T248340 WP_032936429.1 NZ_CP099303:c2360054-2359776 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAITVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10358.10 Da        Isoelectric Point: 8.8094

>AT248340 WP_003836231.1 NZ_CP099303:c2359776-2359492 [Citrobacter freundii]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QC60

References