Detailed information of TA system TA024832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 75906..76516
• Replicon: chromosome
• Accession: NC_014315
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D8K861
• Locus tag: NWAT_RS00340
• Protein ID: WP_013219168.1
• Coordinates: 75906..76187 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NWAT_RS00345
• Protein ID: WP_013219169.1
• Coordinates: 76202..76516 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS00310	70931..71914	-	984	WP_013219161.1	integron integrase	-
NWAT_RS00315	72236..72466	+	231	WP_013219162.1	addiction module protein	-
NWAT_RS00320	72463..72756	+	294	WP_013219163.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NWAT_RS15820	73162..73365	+	204	WP_013219164.1	DUF4160 domain-containing protein	-
NWAT_RS00325	73346..73648	+	303	WP_013219165.1	DUF2442 domain-containing protein	-
NWAT_RS00330	73819..75024	+	1206	WP_013219166.1	FAD-dependent monooxygenase	-
NWAT_RS00335	75281..75688	+	408	WP_049772888.1	hypothetical protein	-
NWAT_RS00340	75906..76187	+	282	WP_013219168.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NWAT_RS00345	76202..76516	+	315	WP_013219169.1	HigA family addiction module antidote protein	Antitoxin
NWAT_RS00350	76620..76808	+	189	WP_013219170.1	type II toxin-antitoxin system HicA family toxin	-
NWAT_RS00355	76817..77032	+	216	WP_013219171.1	type II toxin-antitoxin system HicB family antitoxin	-
NWAT_RS15825	77227..77505	+	279	WP_083781413.1	DUF91 domain-containing protein	-
NWAT_RS16905	77594..77971	+	378	WP_198342168.1	hypothetical protein	-
NWAT_RS16910	78174..78452	+	279	WP_198342169.1	hypothetical protein	-
NWAT_RS16915	78692..79114	+	423	WP_198342170.1	hypothetical protein	-
NWAT_RS00370	79188..79547	+	360	WP_095522183.1	DDE-type integrase/transposase/recombinase	-
NWAT_RS00375	79682..80086	+	405	WP_013219173.1	hypothetical protein	-
NWAT_RS00380	80437..80634	+	198	WP_013219174.1	DUF2283 domain-containing protein	-
NWAT_RS00385	80717..81271	+	555	WP_013219175.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	70931..77089	6158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.29 Da
• Isoelectric Point: 7.8771

>T24832 WP_013219168.1 NC_014315:75906-76187 [Nitrosococcus watsonii C-113]
MIKFFADKRTQELYSKGKSKKFPADVTSRAARKLEYVNLAEQMEDLKVPPDNRLHPLSGNRQGQHAISINDQWRICFRFE
NGDAYEVEVCDYH

Nucleotide

• Length: 282 bp

>T24832 NC_014315:75906-76187 [Nitrosococcus watsonii C-113]
ATGATCAAATTCTTCGCTGACAAACGAACTCAAGAGCTGTACTCCAAAGGCAAGTCAAAGAAGTTTCCTGCGGATGTCAC
ATCGAGAGCTGCCAGAAAACTTGAATATGTGAATCTGGCCGAACAGATGGAGGACTTGAAAGTGCCGCCCGACAATCGCC
TTCATCCACTTTCCGGAAACAGGCAGGGGCAGCACGCAATTTCAATAAATGATCAGTGGCGTATCTGTTTTCGTTTTGAG
AATGGCGATGCGTATGAGGTTGAAGTGTGTGACTACCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11681.40 Da
• Isoelectric Point: 8.7283

>AT24832 WP_013219169.1 NC_014315:76202-76516 [Nitrosococcus watsonii C-113]
MAIPNTAGMQRKPTHPGEMLREDFLPDYGLTVARLAESLGVSRQSVNELLRERRAVSPEMALRLARLFGNSPEFWLNAQR
SVDLWAAAQSVKEEVDRIKPLHAA

Nucleotide

• Length: 315 bp

>AT24832 NC_014315:76202-76516 [Nitrosococcus watsonii C-113]
ATGGCTATTCCTAATACTGCCGGCATGCAGCGCAAGCCGACTCATCCTGGTGAAATGCTGAGAGAGGATTTCCTGCCGGA
CTATGGTTTGACCGTTGCGAGGTTGGCTGAATCTCTGGGTGTCTCTCGCCAGTCAGTTAATGAATTACTGCGTGAGCGCC
GCGCTGTCAGCCCTGAAATGGCGCTCAGGCTTGCCCGTTTGTTTGGTAACTCTCCGGAATTCTGGTTGAACGCGCAGCGA
TCTGTTGATCTTTGGGCGGCGGCTCAGTCTGTTAAGGAAGAAGTGGACCGGATCAAGCCGCTACATGCTGCATAA

Structures

Toxin

• AlphaFold DB: D8K861