Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 34658..35306 Replicon chromosome
Accession NZ_CP099298
Organism Citrobacter freundii strain RHB02-E3-C01

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag NFJ20_RS00170 Protein ID WP_016151211.1
Coordinates 34658..35020 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag NFJ20_RS00175 Protein ID WP_003840660.1
Coordinates 35007..35306 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ20_RS00155 (31117) 31117..32445 + 1329 WP_003023927.1 MFS transporter -
NFJ20_RS00160 (32588) 32588..33979 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
NFJ20_RS00165 (34105) 34105..34557 + 453 WP_003023933.1 DUF1198 family protein -
NFJ20_RS00170 (34658) 34658..35020 + 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFJ20_RS00175 (35007) 35007..35306 + 300 WP_003840660.1 XRE family transcriptional regulator Antitoxin
NFJ20_RS00180 (35425) 35425..36618 + 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
NFJ20_RS00185 (36667) 36667..38049 - 1383 WP_138828044.1 glycoside hydrolase family 1 protein -
NFJ20_RS00190 (38144) 38144..38437 - 294 WP_003840665.1 YicS family protein -
NFJ20_RS00195 (38568) 38568..38984 + 417 WP_044714235.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T248316 WP_016151211.1 NZ_CP099298:34658-35020 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT248316 WP_003840660.1 NZ_CP099298:35007-35306 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References