Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2419967..2420529 Replicon chromosome
Accession NZ_CP099254
Organism Citrobacter freundii strain RHB09-E3-C07

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag NFJ59_RS11485 Protein ID WP_096757033.1
Coordinates 2420251..2420529 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NFJ59_RS11480 Protein ID WP_096757032.1
Coordinates 2419967..2420251 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ59_RS11465 (2415268) 2415268..2418315 + 3048 WP_137398860.1 formate dehydrogenase-N subunit alpha -
NFJ59_RS11470 (2418329) 2418329..2419213 + 885 WP_115258486.1 formate dehydrogenase N subunit beta -
NFJ59_RS11475 (2419206) 2419206..2419862 + 657 WP_096757031.1 formate dehydrogenase-N subunit gamma -
NFJ59_RS11480 (2419967) 2419967..2420251 - 285 WP_096757032.1 HigA family addiction module antitoxin Antitoxin
NFJ59_RS11485 (2420251) 2420251..2420529 - 279 WP_096757033.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFJ59_RS11490 (2420717) 2420717..2422432 - 1716 WP_181630931.1 ABC-F family ATP-binding cassette domain-containing protein -
NFJ59_RS11495 (2422743) 2422743..2424440 - 1698 WP_279278085.1 malate dehydrogenase -
NFJ59_RS11500 (2424620) 2424620..2424760 - 141 WP_181619429.1 stationary-phase-induced ribosome-associated protein -
NFJ59_RS11505 (2424989) 2424989..2425420 + 432 WP_096757037.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10601.05 Da        Isoelectric Point: 9.5320

>T248280 WP_096757033.1 NZ_CP099254:c2420529-2420251 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T248280 NZ_CP099254:c2420529-2420251 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGAGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTAACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTTCAGGCAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10327.05 Da        Isoelectric Point: 8.8094

>AT248280 WP_096757032.1 NZ_CP099254:c2420251-2419967 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Download         Length: 285 bp

>AT248280 NZ_CP099254:c2420251-2419967 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References