Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3833600..3834197 Replicon chromosome
Accession NZ_CP099140
Organism Klebsiella pneumoniae strain RHB28-SO-C05

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NFK38_RS18870 Protein ID WP_004142563.1
Coordinates 3833880..3834197 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NFK38_RS18865 Protein ID WP_004142561.1
Coordinates 3833600..3833887 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK38_RS18840 (NFK38_18825) 3828870..3829985 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
NFK38_RS18845 (NFK38_18830) 3830165..3830746 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
NFK38_RS18850 (NFK38_18835) 3830746..3831114 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
NFK38_RS18855 (NFK38_18840) 3831234..3831887 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NFK38_RS18860 (NFK38_18845) 3832074..3833436 + 1363 Protein_3696 IS3-like element ISKpn1 family transposase -
NFK38_RS18865 (NFK38_18850) 3833600..3833887 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NFK38_RS18870 (NFK38_18855) 3833880..3834197 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFK38_RS18875 (NFK38_18860) 3834382..3835425 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
NFK38_RS18880 (NFK38_18865) 3836095..3836961 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NFK38_RS18885 (NFK38_18870) 3837070..3838497 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T248223 WP_004142563.1 NZ_CP099140:c3834197-3833880 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT248223 WP_004142561.1 NZ_CP099140:c3833887-3833600 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References