Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 333952..334618 Replicon chromosome
Accession NZ_CP099128
Organism Citrobacter freundii strain RHB31-E4-C06

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag NFK47_RS01575 Protein ID WP_003847996.1
Coordinates 334301..334618 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag NFK47_RS01570 Protein ID WP_003837894.1
Coordinates 333952..334248 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK47_RS01555 (331128) 331128..331601 - 474 WP_003023524.1 transcription elongation factor GreB -
NFK47_RS01560 (331827) 331827..332546 + 720 WP_001157751.1 two-component system response regulator OmpR -
NFK47_RS01565 (332543) 332543..333895 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
NFK47_RS01570 (333952) 333952..334248 - 297 WP_003837894.1 NadS family protein Antitoxin
NFK47_RS01575 (334301) 334301..334618 - 318 WP_003847996.1 hypothetical protein Toxin
NFK47_RS01580 (334741) 334741..336363 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
NFK47_RS01585 (336742) 336742..338460 + 1719 WP_046671282.1 DUF4153 domain-containing protein -
NFK47_RS01590 (338570) 338570..339448 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T248203 WP_003847996.1 NZ_CP099128:c334618-334301 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT248203 WP_003837894.1 NZ_CP099128:c334248-333952 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References