Detailed information of TA system TA024817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 354778..355496
• Replicon: plasmid pPag3
• Accession: NC_014258
• Organism: Pantoea vagans C9-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D6YQY9
• Locus tag: PVAG_RS03115
• Protein ID: WP_013196322.1
• Coordinates: 355185..355496 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PVAG_RS03110
• Protein ID: WP_013196321.1
• Coordinates: 354778..355188 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PVAG_RS03090	350550..351557	+	1008	WP_013196317.1	putative FMN-dependent luciferase-like monooxygenase	-
PVAG_RS03095	351554..352645	+	1092	WP_013196318.1	alkylhydroperoxidase domain protein	-
PVAG_RS03100	352657..353796	+	1140	WP_013196319.1	amidohydrolase	-
PVAG_RS03105	353817..354662	+	846	WP_013196320.1	VOC family protein	-
PVAG_RS03110	354778..355188	-	411	WP_013196321.1	helix-turn-helix domain-containing protein	Antitoxin
PVAG_RS03115	355185..355496	-	312	WP_013196322.1	type II toxin-antitoxin system HigB family toxin	Toxin
PVAG_RS03120	355845..358067	-	2223	WP_013196323.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
PVAG_RS03125	358511..359083	+	573	WP_013196324.1	lipid IV(A) palmitoyltransferase PagP	-
PVAG_RS03130	359101..360207	-	1107	WP_041456568.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN / pla	1..529676	529676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12153.97 Da
• Isoelectric Point: 9.3940

>T24817 WP_013196322.1 NC_014258:c355496-355185 [Pantoea vagans C9-1]
MHVISRVPFDEAARKDPNAAAALDLLYRHLRQGDFSGPDDLRALYASLDRMKYRAKWWVIDVSGNHLRVIFFADFERSKI
FIKHICTHAEYDKLVKKYREVTE

Nucleotide

• Length: 312 bp

>T24817 NC_014258:c355496-355185 [Pantoea vagans C9-1]
ATGCATGTTATTTCACGCGTGCCGTTTGACGAGGCGGCCAGAAAAGATCCCAATGCAGCGGCAGCACTGGATCTACTTTA
CCGCCACCTCAGACAGGGCGATTTCAGTGGTCCTGACGATCTCAGGGCGCTTTATGCAAGCCTTGATCGTATGAAGTATC
GCGCTAAATGGTGGGTCATTGACGTCAGTGGCAACCATTTGCGCGTGATATTCTTCGCGGATTTTGAGCGCAGCAAAATC
TTCATCAAGCATATCTGCACTCACGCTGAGTACGACAAGCTTGTTAAGAAATATCGGGAGGTAACCGAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15286.24 Da
• Isoelectric Point: 4.6598

>AT24817 WP_013196321.1 NC_014258:c355188-354778 [Pantoea vagans C9-1]
MNTEAIRLSNQLAEAVPLLAGSNQRHDYEEALELVEYLIEHEPDNPLVDLLCARIAHYEDNDPAMADFNSRQREISTGPG
VLRVLIEQHNLSLSDFENEIGKKSLVSRILSGDRQLTVSHIRALCARFGLSPSLFF

Nucleotide

• Length: 411 bp

>AT24817 NC_014258:c355188-354778 [Pantoea vagans C9-1]
ATGAATACTGAAGCTATCAGGCTGTCAAATCAGCTGGCAGAAGCGGTGCCGCTGCTGGCCGGCAGCAACCAGCGACATGA
TTATGAAGAAGCACTGGAACTGGTCGAATACCTTATTGAACATGAGCCGGATAATCCGCTGGTAGATTTGCTTTGTGCCC
GCATTGCTCATTATGAAGATAATGATCCTGCGATGGCCGACTTTAATAGCCGCCAGCGTGAAATTAGCACCGGACCTGGC
GTCCTGAGGGTACTGATCGAGCAGCACAATCTCAGCCTGAGTGACTTTGAAAATGAAATTGGCAAAAAGTCGCTGGTCAG
CCGAATCCTCAGCGGCGATCGTCAGTTGACCGTGTCACACATACGGGCATTGTGCGCGCGGTTTGGCCTGTCACCATCGT
TGTTTTTTTGA

Structures

Toxin

• AlphaFold DB: D6YQY9