Detailed information of TA system TA024816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 226439..227096
• Replicon: plasmid pPag3
• Accession: NC_014258
• Organism: Pantoea vagans C9-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D6YQL3
• Locus tag: PVAG_RS02560
• Protein ID: WP_013196199.1
• Coordinates: 226725..227096 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D6YQL2
• Locus tag: PVAG_RS02555
• Protein ID: WP_013196198.1
• Coordinates: 226439..226738 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PVAG_RS02535	221682..223124	-	1443	WP_013196193.1	sugar porter family MFS transporter	-
PVAG_RS02540	223446..224348	-	903	WP_013196195.1	LysR family transcriptional regulator	-
PVAG_RS02545	224449..225516	+	1068	WP_013196196.1	SDR family oxidoreductase	-
PVAG_RS02550	225606..226262	+	657	WP_013196197.1	SDR family oxidoreductase	-
PVAG_RS02555	226439..226738	-	300	WP_013196198.1	XRE family transcriptional regulator	Antitoxin
PVAG_RS02560	226725..227096	-	372	WP_013196199.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PVAG_RS02565	227344..228990	-	1647	WP_013196200.1	fumarate hydratase	-
PVAG_RS02570	229147..230655	-	1509	WP_013196201.1	anion permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN / pla	1..529676	529676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14190.15 Da
• Isoelectric Point: 4.8430

>T24816 WP_013196199.1 NC_014258:c227096-226725 [Pantoea vagans C9-1]
MTWEVITRTLFDVWYEKQNDDVQEEIAAYLLILEEIGPQLGRPHVDQVKASEYKNMKELRVQVGGHPFRAFFAFDPERKA
IVLCAGDKKGEDEKLFYKRMIKIADAEFTSHLASPEIKDDGNA

Nucleotide

• Length: 372 bp

>T24816 NC_014258:c227096-226725 [Pantoea vagans C9-1]
ATGACATGGGAAGTCATCACAAGAACGCTGTTTGATGTCTGGTATGAAAAGCAGAACGACGATGTTCAGGAAGAGATTGC
AGCCTACTTGCTGATACTTGAAGAGATTGGCCCTCAATTAGGGCGTCCGCATGTGGATCAGGTTAAAGCGTCTGAATATA
AAAACATGAAAGAGTTGCGAGTACAGGTTGGCGGACATCCATTTCGCGCGTTCTTCGCCTTTGATCCTGAACGCAAGGCG
ATTGTCCTGTGTGCAGGAGATAAAAAAGGTGAAGATGAAAAACTGTTCTACAAACGTATGATTAAAATTGCAGATGCCGA
GTTCACCTCGCATCTGGCATCACCGGAGATTAAAGACGATGGCAACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10971.70 Da
• Isoelectric Point: 10.2633

>AT24816 WP_013196198.1 NC_014258:c226738-226439 [Pantoea vagans C9-1]
MATLKEMMAKRSSQSRTRIESRAAEIRQEITLARLREELSVSQTHLAEVLGVKQPSVAKIENVDNDPKLSTLRRYIAGLG
GELTLDVTLPDGKRIALSL

Nucleotide

• Length: 300 bp

>AT24816 NC_014258:c226738-226439 [Pantoea vagans C9-1]
ATGGCAACGCTTAAAGAAATGATGGCTAAACGCTCCTCGCAGAGCCGTACGCGAATAGAATCCCGTGCTGCTGAAATCCG
TCAGGAGATCACACTTGCCAGACTGCGTGAGGAATTATCTGTTTCACAGACGCATCTAGCTGAAGTTTTAGGCGTCAAAC
AACCTTCTGTTGCTAAAATAGAAAACGTTGATAACGATCCTAAGCTCTCAACGCTGAGGCGCTATATTGCCGGTCTGGGA
GGAGAGCTGACTCTCGATGTCACGTTACCCGACGGCAAGCGTATTGCTCTAAGTCTTTAA

Structures

Toxin

• AlphaFold DB: D6YQL3

Antitoxin

• AlphaFold DB: D6YQL2