Detailed information of TA system TA024806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4407265..4407992
• Replicon: chromosome
• Accession: NC_014228
• Organism: Xenorhabdus nematophila ATCC 19061

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D3VFW9
• Locus tag: XNC1_RS19440
• Protein ID: WP_013185754.1
• Coordinates: 4407681..4407992 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XNC1_RS19435
• Protein ID: WP_013185753.1
• Coordinates: 4407265..4407684 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XNC1_RS19415 (XNC1_4606)	4403498..4404988	+	1491	WP_013185749.1	inorganic phosphate transporter PitA	-
XNC1_RS19420 (XNC1_4608)	4405114..4405443	-	330	WP_010846191.1	universal stress protein UspB	-
XNC1_RS19425 (XNC1_4609)	4405832..4406956	-	1125	Protein_3859	IS701 family transposase	-
XNC1_RS19430 (XNC1_4611)	4406998..4407165	-	168	WP_013185752.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
XNC1_RS19435 (XNC1_4612)	4407265..4407684	-	420	WP_013185753.1	helix-turn-helix domain-containing protein	Antitoxin
XNC1_RS19440 (XNC1_4613)	4407681..4407992	-	312	WP_013185754.1	type II toxin-antitoxin system HigB family toxin	Toxin
XNC1_RS19445 (XNC1_4614)	4408565..4410637	-	2073	WP_013185755.1	phenylacetic acid degradation bifunctional protein PaaZ	-
XNC1_RS19450 (XNC1_4616)	4410966..4411907	+	942	WP_010846184.1	1,2-phenylacetyl-CoA epoxidase subunit A	-
XNC1_RS19455 (XNC1_4617)	4411978..4412265	+	288	WP_013185756.1	1,2-phenylacetyl-CoA epoxidase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12535.52 Da
• Isoelectric Point: 10.0558

>T24806 WP_013185754.1 NC_014228:c4407992-4407681 [Xenorhabdus nematophila ATCC 19061]
MHVISKEPFEQAVRDYPNDANVLITTYKLLKEKNFDSPLELQKLFPSLDNFKHRNKWWVIDIGGNNLRMIAYINFMNKRF
YVKHIVTHKEYDKLTKKYRETKE

Nucleotide

• Length: 312 bp

>T24806 NC_014228:c4407992-4407681 [Xenorhabdus nematophila ATCC 19061]
ATGCACGTTATTTCTAAGGAACCCTTTGAGCAAGCTGTTCGTGATTATCCCAATGATGCAAACGTCCTTATCACGACGTA
TAAGCTGTTAAAAGAAAAGAATTTTGATAGCCCGTTGGAATTACAAAAACTGTTTCCGAGTCTTGATAATTTTAAGCACA
GGAACAAATGGTGGGTAATCGACATAGGTGGCAATAACTTACGGATGATTGCCTATATCAACTTTATGAACAAACGCTTT
TACGTTAAGCACATCGTCACTCACAAAGAATATGACAAATTGACAAAGAAGTATCGGGAGACAAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15251.61 Da
• Isoelectric Point: 5.6660

>AT24806 WP_013185753.1 NC_014228:c4407684-4407265 [Xenorhabdus nematophila ATCC 19061]
MIREPIKALQATTALIAAVPFLGSSASEKDYRDALAMVDYLIENHDESPLINILAAKIAEYEDSNKRFAAFNKAVEVMPT
GVAALRVLMDQHGLSYSDLQEEIGSKSLISQIFSGKRALTIAHIKALSARFDVKPDIFL

Nucleotide

• Length: 420 bp

>AT24806 NC_014228:c4407684-4407265 [Xenorhabdus nematophila ATCC 19061]
ATGATAAGAGAACCTATCAAAGCCCTTCAAGCGACCACGGCTCTGATCGCTGCTGTTCCCTTTTTAGGGAGCAGTGCTTC
TGAAAAGGATTATCGGGATGCACTGGCGATGGTTGATTATCTCATCGAAAACCACGATGAAAGCCCATTAATCAATATAC
TGGCCGCAAAAATAGCGGAGTATGAAGACAGTAACAAACGTTTTGCTGCCTTTAACAAAGCGGTGGAAGTGATGCCAACG
GGTGTCGCCGCGCTGCGTGTATTGATGGATCAACATGGCTTGTCATATTCAGATTTGCAAGAAGAAATAGGCTCAAAGTC
ACTGATCAGCCAAATTTTTTCGGGAAAACGAGCATTAACCATTGCACATATCAAGGCACTTTCTGCCCGTTTTGATGTGA
AGCCGGATATCTTTCTCTGA

Structures

Toxin

• AlphaFold DB: D3VFW9