Detailed information of TA system TA024791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 100845..101495
• Replicon: chromosome
• Accession: NC_014228
• Organism: Xenorhabdus nematophila ATCC 19061

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N1NNR7
• Locus tag: XNC1_RS00460
• Protein ID: WP_010847562.1
• Coordinates: 100845..101153 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XNC1_RS00465
• Protein ID: WP_010847563.1
• Coordinates: 101199..101495 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XNC1_RS23180 (XNC1_0097)	96629..96766	+	138	WP_156148016.1	hypothetical protein	-
XNC1_RS00440 (XNC1_0098)	96744..96962	+	219	Protein_83	Fic family protein	-
XNC1_RS00445 (XNC1_0099)	97098..98084	-	987	WP_113967570.1	sulfate ABC transporter substrate-binding protein	-
XNC1_RS00450 (XNC1_0100)	98387..99364	-	978	WP_013183083.1	6-phosphofructokinase	-
XNC1_RS00455 (XNC1_0101)	99600..100499	-	900	WP_010847560.1	CDF family cation-efflux transporter FieF	-
XNC1_RS00460 (XNC1_0103)	100845..101153	+	309	WP_010847562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XNC1_RS00465 (XNC1_0104)	101199..101495	+	297	WP_010847563.1	helix-turn-helix domain-containing protein	Antitoxin
XNC1_RS00475 (XNC1_0105)	101830..102246	-	417	WP_010847564.1	type II toxin-antitoxin system HicB family antitoxin	-
XNC1_RS00480 (XNC1_0106)	102301..102475	-	175	Protein_90	type II toxin-antitoxin system HicA family toxin	-
XNC1_RS00485 (XNC1_0107)	102607..103050	-	444	WP_010847567.1	hypothetical protein	-
XNC1_RS00490 (XNC1_0108)	103423..103923	-	501	WP_010847568.1	Spy/CpxP family protein refolding chaperone	-
XNC1_RS00495 (XNC1_0109)	104076..104768	+	693	WP_013183086.1	envelope stress response regulator transcription factor CpxR	-
XNC1_RS00500 (XNC1_0110)	104765..106147	+	1383	WP_013183087.1	envelope stress sensor histidine kinase CpxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-92)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11887.92 Da
• Isoelectric Point: 10.5016

>T24791 WP_010847562.1 NC_014228:100845-101153 [Xenorhabdus nematophila ATCC 19061]
VYKRQVFSRERAELLTDDEFRHLQEHLLKNHEQGSTISATGGCKKIRLQREGMGKRGGVRIIYYTIIKTGMLYLLLIYPK
NKKDDLTPKQKEVLKAISNKLQ

Nucleotide

• Length: 309 bp

>T24791 NC_014228:100845-101153 [Xenorhabdus nematophila ATCC 19061]
GTGTATAAGAGACAGGTATTCAGTAGGGAACGGGCAGAGTTGTTAACCGACGATGAATTTAGACACTTACAGGAACATTT
ACTAAAGAATCATGAACAAGGCAGTACCATTAGTGCTACTGGAGGATGCAAAAAGATTCGCTTGCAGCGGGAAGGAATGG
GAAAACGTGGCGGTGTCAGAATTATCTACTACACCATCATTAAAACCGGAATGCTCTATCTTCTTTTGATTTACCCTAAA
AATAAAAAAGATGACTTAACCCCAAAACAAAAAGAAGTACTTAAAGCCATCAGTAATAAATTGCAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11083.80 Da
• Isoelectric Point: 7.3210

>AT24791 WP_010847563.1 NC_014228:101199-101495 [Xenorhabdus nematophila ATCC 19061]
MDKKLFDDLIESMNQMVAIDKGELSVPAENIHRHRLPDVKKLRETFGLKQSEFAEAVGVSASLIQSWEQHRRTPSGSSLK
LLKMLERNPALINELSAL

Nucleotide

• Length: 297 bp

>AT24791 NC_014228:101199-101495 [Xenorhabdus nematophila ATCC 19061]
ATGGACAAAAAACTTTTTGATGATTTGATTGAAAGCATGAATCAAATGGTAGCGATTGATAAAGGGGAACTTTCTGTTCC
TGCTGAAAATATTCATCGTCATCGTCTGCCTGATGTAAAAAAACTACGCGAAACCTTTGGGTTAAAGCAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTGCATCCTTGATCCAAAGCTGGGAGCAACACCGCCGTACTCCTTCTGGTTCATCACTTAAA
TTGCTTAAAATGCTTGAACGTAATCCTGCGCTGATTAATGAATTAAGCGCGCTTTAA

Structures

Toxin

• AlphaFold DB: N1NNR7