Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 67894..68559 Replicon plasmid pYR4
Accession NZ_CP098717
Organism Yersinia ruckeri strain NVI-11294

Toxin (Protein)


Gene name higB Uniprot ID A0A380S9H4
Locus tag ND011_RS17840 Protein ID WP_038251855.1
Coordinates 68236..68559 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ND011_RS17835 Protein ID WP_004720859.1
Coordinates 67894..68190 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND011_RS17810 (ND011_17800) 63191..65116 - 1926 WP_004720847.1 relaxase/mobilization nuclease domain-containing protein -
ND011_RS17815 (ND011_17805) 65118..65462 - 345 WP_004720848.1 plasmid mobilization protein MobA -
ND011_RS17820 (ND011_17810) 65731..66054 + 324 WP_004720850.1 hypothetical protein -
ND011_RS17825 (ND011_17815) 66201..66371 - 171 WP_004720852.1 DUF5983 family protein -
ND011_RS17830 (ND011_17820) 66593..67406 - 814 Protein_76 DUF932 domain-containing protein -
ND011_RS17835 (ND011_17825) 67894..68190 - 297 WP_004720859.1 NadS family protein Antitoxin
ND011_RS17840 (ND011_17830) 68236..68559 - 324 WP_038251855.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ND011_RS17845 (ND011_17835) 68711..68944 + 234 WP_038251853.1 hypothetical protein -
ND011_RS17850 (ND011_17840) 68952..69449 - 498 WP_126940174.1 hypothetical protein -
ND011_RS17855 (ND011_17845) 69446..69805 - 360 WP_126940175.1 DUF3085 domain-containing protein -
ND011_RS17860 (ND011_17850) 70564..71376 - 813 WP_238692894.1 N-6 DNA methylase -
ND011_RS17865 (ND011_17855) 71420..71566 - 147 WP_126940177.1 succinate dehydrogenase flavoprotein subunit -
ND011_RS17870 (ND011_17860) 71652..72008 - 357 WP_126940178.1 hypothetical protein -
ND011_RS17875 (ND011_17865) 72762..72962 - 201 WP_126940179.1 hypothetical protein -
ND011_RS17880 (ND011_17870) 73005..73511 - 507 WP_126940180.1 antirestriction protein ArdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..115590 115590


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(46-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-82)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12416.31 Da        Isoelectric Point: 8.8371

>T247883 WP_038251855.1 NZ_CP098717:c68559-68236 [Yersinia ruckeri]
MEYLEFIETPVFSRRRAELLPDDDFRALQEHLLKNHDLGDTISKTGGCKKIRWSREGMGKRGGVRVIYYVITKSGRLYLL
LVYPKNEKDDLTEAEKAVLKSISQQIE

Download         Length: 324 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11027.77 Da        Isoelectric Point: 6.7264

>AT247883 WP_004720859.1 NZ_CP098717:c68190-67894 [Yersinia ruckeri]
MKTELFNDLVESMQQMVAIEKGTLSVPAENIHRHRLPDVKSMREASGLKQNEFADVVGVSASLLQSWEQHRRIPSGSSLK
LLMMIERDPAVIDALRTL

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A380S9H4

Antitoxin

Source ID Structure

References