Detailed information of TA system TA024755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_31
• Location: 83456..84060
• Replicon: chromosome
• Accession: NC_014125
• Organism: Legionella pneumophila 2300/99 Alcoy

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A128WF47
• Locus tag: LPA_RS00395
• Protein ID: WP_011212715.1
• Coordinates: 83456..83779 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LPA_RS00400
• Protein ID: WP_011212716.1
• Coordinates: 83776..84060 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPA_RS00370	78595..79899	+	1305	WP_011945267.1	ATP-grasp domain-containing protein	-
LPA_RS00375	79920..80510	+	591	WP_011945268.1	alpha/beta hydrolase	-
LPA_RS00380	80494..81441	+	948	WP_011945269.1	DMT family transporter	-
LPA_RS00385	81541..81888	+	348	Protein_74	transposase family protein	-
LPA_RS00390	82149..83372	+	1224	WP_011945271.1	site-specific integrase	-
LPA_RS00395	83456..83779	+	324	WP_011212715.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LPA_RS00400	83776..84060	+	285	WP_011212716.1	helix-turn-helix domain-containing protein	Antitoxin
LPA_RS00405	84242..85057	+	816	WP_011945272.1	hypothetical protein	-
LPA_RS00410	85832..87244	-	1413	WP_011945274.1	hypothetical protein	-
LPA_RS00415	87275..87883	-	609	WP_011945275.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	81541..81918	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12144.36 Da
• Isoelectric Point: 9.7878

>T24755 WP_011212715.1 NC_014125:83456-83779 [Legionella pneumophila 2300/99 Alcoy]
MSWIVEFYNESVEEAILTMPPKIQARMIKLLELIETHGANLGPPHTEAMGDGLFEIRAKAQEGIGRSLYCYMKGKHIVVL
HAFVKKSAKTPKPDLQLALKRKREVEK

Nucleotide

• Length: 324 bp

>T24755 NC_014125:83456-83779 [Legionella pneumophila 2300/99 Alcoy]
ATGTCTTGGATTGTTGAATTTTATAATGAGTCTGTTGAAGAGGCCATACTTACAATGCCGCCAAAAATTCAGGCTCGAAT
GATTAAACTTTTGGAATTGATTGAAACACATGGCGCTAATCTTGGGCCTCCTCATACAGAAGCGATGGGTGATGGGTTAT
TTGAGATTCGCGCAAAAGCTCAAGAAGGAATCGGGCGATCGCTTTATTGCTATATGAAGGGAAAGCATATTGTGGTTTTG
CATGCATTTGTAAAGAAAAGTGCAAAAACTCCAAAACCAGATTTGCAATTAGCTCTCAAAAGAAAGCGGGAGGTAGAAAA
ATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.22 Da
• Isoelectric Point: 9.8787

>AT24755 WP_011212716.1 NC_014125:83776-84060 [Legionella pneumophila 2300/99 Alcoy]
MMKLKTLKAKALKNPEVKEAYDALESEFALIDALLTMRQKAGLTQEELAERMGTKKGNISRLEKGNTNPSWNTLEKYAHA
CGFDISMAVKRIAS

Nucleotide

• Length: 285 bp

>AT24755 NC_014125:83776-84060 [Legionella pneumophila 2300/99 Alcoy]
ATGATGAAACTTAAAACATTAAAGGCTAAAGCGTTAAAAAATCCTGAAGTAAAAGAAGCATACGATGCTTTGGAATCCGA
ATTTGCATTGATCGATGCGTTACTGACGATGCGTCAAAAAGCTGGATTAACTCAAGAAGAGTTAGCTGAGCGAATGGGGA
CAAAAAAAGGGAATATTTCTCGACTGGAAAAAGGAAATACTAATCCAAGCTGGAATACTTTAGAGAAATATGCACATGCG
TGTGGGTTTGATATTTCAATGGCAGTAAAGAGAATCGCTTCATAA

Structures

Toxin

• AlphaFold DB: A0A128WF47