Detailed information of TA system TA024754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5233206..5233808
• Replicon: chromosome
• Accession: NC_014121
• Organism: Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: ECL_RS25400
• Protein ID: WP_013099360.1
• Coordinates: 5233497..5233808 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECL_RS25395
• Protein ID: WP_013099359.1
• Coordinates: 5233206..5233496 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECL_RS25380	5230703..5231605	+	903	WP_013099356.1	formate dehydrogenase subunit beta	-
ECL_RS25385	5231602..5232237	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
ECL_RS25390	5232234..5233163	+	930	WP_013099358.1	formate dehydrogenase accessory protein FdhE	-
ECL_RS25395	5233206..5233496	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
ECL_RS25400	5233497..5233808	-	312	WP_013099360.1	hypothetical protein	Toxin
ECL_RS25405	5234038..5234955	+	918	WP_013099361.1	alpha/beta hydrolase	-
ECL_RS25410	5234968..5235909	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
ECL_RS25415	5235954..5236391	-	438	WP_013099363.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECL_RS25420	5236388..5237269	-	882	WP_013099364.1	virulence factor BrkB family protein	-
ECL_RS25425	5237263..5237862	-	600	WP_013099365.1	glucose-1-phosphatase	-
ECL_RS25430	5237982..5238782	-	801	WP_013099366.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T24754 WP_013099360.1 NC_014121:c5233808-5233497 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T24754 NC_014121:c5233808-5233497 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAATTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT24754 WP_013099359.1 NC_014121:c5233496-5233206 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT24754 NC_014121:c5233496-5233206 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGACGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAACGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02