Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3231545..3232194 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag ECL_RS15610 Protein ID WP_013097692.1
Coordinates 3231545..3231907 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag ECL_RS15615 Protein ID WP_013097693.1
Coordinates 3231895..3232194 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS15590 3226879..3227850 - 972 WP_013097688.1 LacI family transcriptional regulator -
ECL_RS15595 3227926..3229068 - 1143 WP_013097689.1 Gfo/Idh/MocA family oxidoreductase -
ECL_RS15600 3229061..3230086 - 1026 WP_013097690.1 sugar phosphate isomerase/epimerase -
ECL_RS15605 3230102..3231340 - 1239 WP_013097691.1 MFS transporter -
ECL_RS15610 3231545..3231907 + 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECL_RS15615 3231895..3232194 + 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
ECL_RS15620 3232320..3233708 - 1389 WP_013097694.1 phenylalanine transporter -
ECL_RS15625 3233811..3235784 - 1974 WP_013097695.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3221656..3232194 10538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T24750 WP_013097692.1 NC_014121:3231545-3231907 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T24750 NC_014121:3231545-3231907 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT24750 WP_013097693.1 NC_014121:3231895-3232194 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT24750 NC_014121:3231895-3232194 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTGATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCTGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACAGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTGACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References