247421

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 367119..367785 Replicon chromosome
Accession NZ_CP098330
Organism Citrobacter freundii strain 59174

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag NB664_RS01710 Protein ID WP_003847996.1
Coordinates 367468..367785 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag NB664_RS01705 Protein ID WP_003837894.1
Coordinates 367119..367415 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB664_RS01685 (363095) 363095..363568 - 474 WP_003023524.1 transcription elongation factor GreB -
NB664_RS01690 (363811) 363811..364791 + 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -
NB664_RS01695 (364994) 364994..365713 + 720 WP_001157751.1 two-component system response regulator OmpR -
NB664_RS01700 (365710) 365710..367062 + 1353 WP_250791508.1 two-component system sensor histidine kinase EnvZ -
NB664_RS01705 (367119) 367119..367415 - 297 WP_003837894.1 NadS family protein Antitoxin
NB664_RS01710 (367468) 367468..367785 - 318 WP_003847996.1 hypothetical protein Toxin
NB664_RS01715 (367908) 367908..369530 - 1623 WP_044701411.1 phosphoenolpyruvate carboxykinase (ATP) -
NB664_RS01720 (369909) 369909..371627 + 1719 WP_047715944.1 DUF4153 domain-containing protein -
NB664_RS01725 (371737) 371737..372615 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 363811..364791 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T247421 WP_003847996.1 NZ_CP098330:c367785-367468 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT247421 WP_003837894.1 NZ_CP098330:c367415-367119 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References