Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2407368..2407930 Replicon chromosome
Accession NZ_CP098231
Organism Escherichia coli strain XJ34

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KFZ60_RS11650 Protein ID WP_000605676.1
Coordinates 2407368..2407646 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KFZ60_RS11655 Protein ID WP_000781370.1
Coordinates 2407646..2407930 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ60_RS11625 (2402954) 2402954..2403385 - 432 WP_032280863.1 peroxiredoxin OsmC -
KFZ60_RS11630 (2403731) 2403731..2403946 + 216 WP_000495766.1 biofilm-dependent modulation protein -
KFZ60_RS11635 (2404048) 2404048..2404185 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KFZ60_RS11640 (2404341) 2404341..2406038 + 1698 WP_088376254.1 malate dehydrogenase -
KFZ60_RS11645 (2406172) 2406172..2407182 + 1011 WP_088376255.1 alcohol dehydrogenase AdhP -
KFZ60_RS11650 (2407368) 2407368..2407646 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFZ60_RS11655 (2407646) 2407646..2407930 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KFZ60_RS11660 (2408158) 2408158..2408811 - 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
KFZ60_RS11665 (2408804) 2408804..2409688 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
KFZ60_RS11670 (2409701) 2409701..2412748 - 3048 WP_012767733.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T247301 WP_000605676.1 NZ_CP098231:2407368-2407646 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT247301 WP_000781370.1 NZ_CP098231:2407646-2407930 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References