Detailed information of TA system TA247244

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29733..30151
• Replicon: plasmid pZ0117EC0005-2
• Accession: NZ_CP098225
• Organism: Escherichia coli strain Z0117EC0005

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NBY12_RS24000
• Protein ID: WP_223412041.1
• Coordinates: 30029..30151 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29733..29937 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NBY12_RS23965	25433..25954	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
NBY12_RS23970	26010..26243	+	234	WP_000005971.1	DUF905 domain-containing protein	-
NBY12_RS23975	26307..28265	+	1959	WP_233108850.1	ParB/RepB/Spo0J family partition protein	-
NBY12_RS23980	28320..28754	+	435	WP_021572430.1	conjugation system SOS inhibitor PsiB	-
NBY12_RS23985	28751..29470	+	720	WP_100774605.1	plasmid SOS inhibition protein A	-
NBY12_RS23990	29467..29781	+	315	WP_000872086.1	hypothetical protein	-
-	29730..29937	+	208	NuclAT_0	-	-
-	29730..29937	+	208	NuclAT_0	-	-
-	29730..29937	+	208	NuclAT_0	-	-
-	29730..29937	+	208	NuclAT_0	-	-
-	29733..29937	-	205	-	-	Antitoxin
NBY12_RS23995	29971..30084	+	114	Protein_42	DUF5431 family protein	-
NBY12_RS24000	30029..30151	+	123	WP_223412041.1	Hok/Gef family protein	Toxin
NBY12_RS24005	30565..31068	+	504	Protein_44	IS66-like element accessory protein TnpA	-
NBY12_RS24010	31336..31659	-	324	WP_025733244.1	hypothetical protein	-
NBY12_RS24015	31620..32069	-	450	WP_013362817.1	hypothetical protein	-
NBY12_RS24020	32699..33436	-	738	WP_250777852.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
NBY12_RS24025	33562..33657	-	96	WP_013362819.1	23S rRNA methyltransferase attenuation leader peptide	-
NBY12_RS24030	33792..34496	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
NBY12_RS24035	34549..34785	-	237	Protein_50	DUF3330 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / erm(B) / aadA5 / qacE / sul1 / mph(A) / aac(3)-IId / blaTEM-1B	-	1..57370	57370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4560.33 Da
• Isoelectric Point: 8.2774

>T247244 WP_223412041.1 NZ_CP098225:30029-30151 [Escherichia coli]
IVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 205 bp

>AT247244 NZ_CP098225:c29937-29733 [Escherichia coli]
AGACATACAGGGGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTCTGCCTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCAC
TTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin