Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4097212..4097939 Replicon chromosome
Accession NZ_CP098223
Organism Escherichia coli strain Z0117EC0005

Toxin (Protein)


Gene name higB Uniprot ID A0A2K3TXF2
Locus tag NBY12_RS19550 Protein ID WP_032303813.1
Coordinates 4097625..4097939 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NBY12_RS19545 Protein ID WP_000560266.1
Coordinates 4097212..4097628 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY12_RS19535 (4092573) 4092573..4094924 + 2352 WP_032303811.1 alpha-glucosidase -
NBY12_RS19540 (4095149) 4095149..4097167 + 2019 WP_050009868.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NBY12_RS19545 (4097212) 4097212..4097628 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NBY12_RS19550 (4097625) 4097625..4097939 - 315 WP_032303813.1 type II toxin-antitoxin system toxin HigB Toxin
NBY12_RS19555 (4098195) 4098195..4099331 - 1137 WP_000018675.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NBY12_RS19560 (4099416) 4099416..4099919 + 504 WP_001295542.1 M48 family metallopeptidase -
NBY12_RS19565 (4099997) 4099997..4100689 + 693 WP_032303814.1 vancomycin high temperature exclusion protein -
NBY12_RS19570 (4100768) 4100768..4101754 + 987 WP_103253354.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12119.06 Da        Isoelectric Point: 9.9056

>T247235 WP_032303813.1 NZ_CP098223:c4097939-4097625 [Escherichia coli]
MHLITQKELKDAAEKYPQHKTELGALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT247235 WP_000560266.1 NZ_CP098223:c4097628-4097212 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K3TXF2

Antitoxin

Source ID Structure

References