Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2390186..2390748 Replicon chromosome
Accession NZ_CP098223
Organism Escherichia coli strain Z0117EC0005

Toxin (Protein)


Gene name higB Uniprot ID A0A2K3TT64
Locus tag NBY12_RS11435 Protein ID WP_032303016.1
Coordinates 2390470..2390748 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag NBY12_RS11430 Protein ID WP_000781370.1
Coordinates 2390186..2390470 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY12_RS11415 (2385545) 2385545..2388592 + 3048 WP_162754249.1 formate dehydrogenase-N subunit alpha -
NBY12_RS11420 (2388605) 2388605..2389489 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
NBY12_RS11425 (2389482) 2389482..2390135 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
NBY12_RS11430 (2390186) 2390186..2390470 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
NBY12_RS11435 (2390470) 2390470..2390748 - 279 WP_032303016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY12_RS11440 (2390934) 2390934..2391944 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
NBY12_RS11445 (2392079) 2392079..2393776 - 1698 WP_032303017.1 malate dehydrogenase -
NBY12_RS11450 (2393933) 2393933..2394070 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
NBY12_RS11455 (2394172) 2394172..2394387 - 216 WP_000495766.1 biofilm-dependent modulation protein -
NBY12_RS11460 (2394732) 2394732..2395163 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T247228 WP_032303016.1 NZ_CP098223:c2390748-2390470 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADLDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT247228 WP_000781370.1 NZ_CP098223:c2390470-2390186 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K3TT64

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References