Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1351477..1352156 Replicon chromosome
Accession NZ_CP098219
Organism Escherichia coli strain Z0117EC0013

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag NBY13_RS06335 Protein ID WP_000057523.1
Coordinates 1351477..1351779 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NBY13_RS06340 Protein ID WP_000806442.1
Coordinates 1351815..1352156 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY13_RS06310 (1346853) 1346853..1348505 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
NBY13_RS06315 (1348543) 1348543..1349046 - 504 WP_000667000.1 hypothetical protein -
NBY13_RS06320 (1349043) 1349043..1349843 - 801 WP_000439798.1 hypothetical protein -
NBY13_RS06325 (1349867) 1349867..1350346 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NBY13_RS06330 (1350550) 1350550..1351344 - 795 WP_000365147.1 TraB/GumN family protein -
NBY13_RS06335 (1351477) 1351477..1351779 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY13_RS06340 (1351815) 1351815..1352156 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NBY13_RS06345 (1352214) 1352214..1354718 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
NBY13_RS06350 (1354980) 1354980..1355912 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T247185 WP_000057523.1 NZ_CP098219:1351477-1351779 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT247185 WP_000806442.1 NZ_CP098219:1351815-1352156 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References