Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4423391..4424190 Replicon chromosome
Accession NZ_CP098217
Organism Escherichia coli strain Z0117EC0032

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NBY14_RS21030 Protein ID WP_000347270.1
Coordinates 4423726..4424190 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NBY14_RS21025 Protein ID WP_001551693.1
Coordinates 4423391..4423726 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY14_RS21010 (4419176) 4419176..4419946 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
NBY14_RS21015 (4419962) 4419962..4421296 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NBY14_RS21020 (4421671) 4421671..4423242 + 1572 WP_001273738.1 galactarate dehydratase -
NBY14_RS21025 (4423391) 4423391..4423726 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NBY14_RS21030 (4423726) 4423726..4424190 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NBY14_RS21035 (4424245) 4424245..4425054 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NBY14_RS21040 (4425303) 4425303..4426583 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NBY14_RS21045 (4426606) 4426606..4427079 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NBY14_RS21050 (4427090) 4427090..4427869 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NBY14_RS21055 (4427859) 4427859..4428737 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NBY14_RS21060 (4428755) 4428755..4429189 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4415190..4424190 9000


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T247176 WP_000347270.1 NZ_CP098217:4423726-4424190 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT247176 WP_001551693.1 NZ_CP098217:4423391-4423726 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References