Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3860030..3860724 Replicon chromosome
Accession NZ_CP098214
Organism Escherichia coli strain Z0117EC0036

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag NBY15_RS18965 Protein ID WP_001263500.1
Coordinates 3860030..3860428 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NBY15_RS18970 Protein ID WP_000554758.1
Coordinates 3860431..3860724 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY15_RS18940 (3855396) 3855396..3855854 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
NBY15_RS18945 (3856115) 3856115..3857572 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
NBY15_RS18950 (3857629) 3857629..3858243 - 615 WP_000602123.1 peptide chain release factor H -
NBY15_RS18955 (3858240) 3858240..3859379 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
NBY15_RS18960 (3859568) 3859568..3860020 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
NBY15_RS18965 (3860030) 3860030..3860428 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NBY15_RS18970 (3860431) 3860431..3860724 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NBY15_RS18975 (3860776) 3860776..3861831 - 1056 WP_001226168.1 DNA polymerase IV -
NBY15_RS18980 (3861902) 3861902..3862687 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
NBY15_RS18985 (3862659) 3862659..3864371 + 1713 Protein_3727 flagellar biosynthesis protein FlhA -
NBY15_RS18990 (3864450) 3864450..3864695 + 246 WP_133258463.1 hypothetical protein -
NBY15_RS18995 (3864690) 3864690..3865187 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3860030..3880383 20353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T247135 WP_001263500.1 NZ_CP098214:c3860428-3860030 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT247135 WP_000554758.1 NZ_CP098214:c3860724-3860431 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References