Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3625498..3626177 Replicon chromosome
Accession NZ_CP098214
Organism Escherichia coli strain Z0117EC0036

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag NBY15_RS17875 Protein ID WP_000057524.1
Coordinates 3625875..3626177 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NBY15_RS17870 Protein ID WP_000806442.1
Coordinates 3625498..3625839 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY15_RS17860 (3621742) 3621742..3622674 - 933 WP_000883041.1 glutaminase A -
NBY15_RS17865 (3622936) 3622936..3625440 + 2505 WP_021513535.1 copper-exporting P-type ATPase CopA -
NBY15_RS17870 (3625498) 3625498..3625839 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NBY15_RS17875 (3625875) 3625875..3626177 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NBY15_RS17880 (3626310) 3626310..3627104 + 795 WP_000365177.1 TraB/GumN family protein -
NBY15_RS17885 (3627308) 3627308..3627787 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NBY15_RS17890 (3627811) 3627811..3628611 + 801 WP_000439798.1 hypothetical protein -
NBY15_RS17895 (3628608) 3628608..3629111 + 504 WP_000667000.1 hypothetical protein -
NBY15_RS17900 (3629149) 3629149..3630801 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T247133 WP_000057524.1 NZ_CP098214:c3626177-3625875 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT247133 WP_000806442.1 NZ_CP098214:c3625839-3625498 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References