Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4376618..4377417 Replicon chromosome
Accession NZ_CP098210
Organism Escherichia coli strain Z0117EC0051

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NBY17_RS20845 Protein ID WP_000347270.1
Coordinates 4376953..4377417 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NBY17_RS20840 Protein ID WP_001551693.1
Coordinates 4376618..4376953 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY17_RS20825 (4372403) 4372403..4373173 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
NBY17_RS20830 (4373189) 4373189..4374523 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NBY17_RS20835 (4374898) 4374898..4376469 + 1572 WP_001273738.1 galactarate dehydratase -
NBY17_RS20840 (4376618) 4376618..4376953 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NBY17_RS20845 (4376953) 4376953..4377417 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NBY17_RS20850 (4377472) 4377472..4378281 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NBY17_RS20855 (4378530) 4378530..4379810 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NBY17_RS20860 (4379833) 4379833..4380306 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NBY17_RS20865 (4380317) 4380317..4381096 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NBY17_RS20870 (4381086) 4381086..4381964 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NBY17_RS20875 (4381982) 4381982..4382416 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island sul2 / aph(6)-Id / aph(3'')-Ib - 4350882..4377417 26535


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T247094 WP_000347270.1 NZ_CP098210:4376953-4377417 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT247094 WP_001551693.1 NZ_CP098210:4376618-4376953 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References